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PhosphoSitePlus® Resource

  • Additional protein information
  • Analytical tools


Product Includes Quantity Applications Reactivity MW(kDa) Isotype
cdc2 (POH1) Mouse mAb 9116 20 µl
Western Blotting Immunoprecipitation Immunofluorescence
H Mk 34 Mouse IgG2a
CDK2 (78B2) Rabbit mAb 2546 20 µl
Western Blotting Immunoprecipitation Flow Cytometry
H M R Mk 33 Rabbit 
CDK4 (D9G3E) Rabbit mAb 12790 20 µl
Western Blotting Immunohistochemistry Immunofluorescence Flow Cytometry
H Mk 30 Rabbit IgG
CDK6 (D4S8S) Rabbit mAb 13331 20 µl
Western Blotting Immunoprecipitation
H Mk 36 Rabbit IgG
CDK7 (MO1) Mouse mAb 2916 20 µl
Western Blotting Immunoprecipitation Immunohistochemistry
H R 40 Mouse IgG2b
CDK9 (C12F7) Rabbit mAb 2316 20 µl
Western Blotting Immunoprecipitation Immunohistochemistry Immunofluorescence Flow Cytometry
H M R Hm Mk B Dg 42, 55 Rabbit 
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
Western Blotting
Anti-mouse IgG, HRP-linked Antibody 7076 100 µl
Western Blotting

Product Description

The CDK Antbody Sampler Kit provides and economical means of evaluating Cdk proteins. The kit contains enough primary and secondary antibodies to perform two western blot experiments.

Specificity / Sensitivity

Each antibody in the CDK Antibody Sampler Kit detects endogenous levels of its respective target protein and does not cross-react with other family members.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant human cdc2 fusion protein, synthetic peptides corresponding to residues of human CDK2 and recombinant human CDK7, and residues near the carboxy terminus of human CDK4, human CDK6, and human CDK9 proteins.

Cyclin-dependent kinases (CDKs) are the core effectors of cell cycle progression. CDK activity is regulated through association with their cyclin partners and cyclin-dependent kinase inhibitors (CKIs) as well as by activating and inhibitory phosphorylation events. Inhibition is mediated by Wee1 and Myt1 kinases that target residues at the amino terminus of CDK1 (1,2). Dephosphorylation of these residues by cdc25 phosphatase leads to activation of CDK kinase activity (3). The CDK7/cyclinH complex is the ubiquitous mammalian CDK-activating kinase (CAK) that phosphorylates a conserved threonine residue in the T-loop domain of CDKs. The carboxy-terminal domain of RNA polymerase II is also a target of CAK as well as CDK9/cyclinT (4,5). CDK4/6 associate with cyclinD and phosphorylate retinoblastoma protein and initiate progression through the restriction point in G1 (6). CDK2 associates with cyclinE in early S phase and cyclinA later in G2. CDK1/cyclinB regulates the initiation of mitotic events (7).

1.  Fattaey, A. and Booher, R.N. (1997) Prog Cell Cycle Res 3, 233-40.

2.  Jessus, C. and Ozon, R. (1995) Prog. Cell Cycle Res. 1, 215-228.

3.  De Falco, G. and Giordano, A. Cancer Biol Ther 1, 342-7.

4.  Kaldis, P. (1999) Cell Mol Life Sci 55, 284-96.

5.  Booher, R.N. et al. (1997) J Biol Chem 272, 22300-6.

6.  Morgan, D.O. (1997) Annu Rev Cell Dev Biol 13, 261-91.

7.  Sherr, C.J. (1995) Trends Biochem Sci 20, 187-90.

Entrez-Gene Id 983 , 1017 , 1019 , 1021 , 1022 , 1025
Swiss-Prot Acc. P06493 , P24941 , P11802 , Q00534 , P50613 , P50750

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.

CDK Antibody Sampler Kit