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877-616-CELL (2355)

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Support:

877-678-TECH (8324)

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For Research Use Only. Not for Use in Diagnostic Procedures.

Product Includes Product # Quantity Mol. Wt Isotype/Source
Geminin (E5Q9S) Rabbit Monoclonal Antibody5250820 µl25 kDaRabbit IgG
CDT1 (D10F11) Rabbit Monoclonal Antibody806420 µl65 kDaRabbit IgG
Thymidine Kinase 1 (E2H7Z) Rabbit Monoclonal Antibody2875520 µl26 kDaRabbit IgG
Phospho-Histone H3 (Ser10) (D7N8E) Rabbit Monoclonal Antibody5334820 µl17 kDaRabbit IgG
Cyclin A2 (E1D9T) Rabbit Monoclonal Antibody9150020 µl55 kDaRabbit IgG
Cyclin B1 (D5C10) Rabbit Monoclonal Antibody1223120 µl55 kDaRabbit IgG
Cyclin E1 (D7T3U) Rabbit Monoclonal Antibody2080820 µl48 kDaRabbit IgG
Phospho-cdc2 (Tyr15) (10A11) Rabbit Monoclonal Antibody453920 µl34 kDaRabbit
Anti-rabbit IgG, HRP-linked Antibody7074100 µlGoat

Please visit cellsignal.com for individual component applications, species cross-reactivity, dilutions, protocols, and additional product information.

Description

The Cell Cycle Phase Determination Antibody Sampler Kit provides an economical means of detecting total proteins or post-translational modifications present in cells at various phases of the cell cycle. Geminin is degraded in G1 phase, while CDT1 is degraded in S, G2, and M phases. Thymidine Kinase 1 accumulates in G1 phase, peaks in S phase, and is degraded before cell division. Phospho-Histone H3 (Ser10) is present only in M phase, while Phospho-cdc2 (Tyr15) is absent in M phase. Cyclins A2, B1, and E1 peak at G2 phase, late G2/M phase, and late G1/early S phase, respectively. The kit includes enough antibodies to perform two western blot experiments with each primary antibody.

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Background

The entry of eukaryotic cells into mitosis is regulated by cdc2/CDK1 kinase activation, a process controlled at several steps including cyclin B1 nuclear accumulation and binding, and phosphorylation of cdc2/CDK1 at Thr161 (1). At the end of mitosis, cyclin B1 is targeted for degradation by the anaphase-promoting complex (APC), allowing for cell cycle progression (2). A critical regulatory step in activating cdc2 during progression into mitosis is dephosphorylation of cdc2/CDK1 at Thr14 and Tyr15 (3).
Phosphorylation of Histone H3 at Ser10 is tightly correlated with chromosome condensation during both mitosis and meiosis (4).
Overcoming the G1/S checkpoint to commence DNA replication requires cyclin E, traversing the G2/M checkpoint to initiate mitosis requires cyclin B, and cyclin A is required for both S-phase and M-phase (5). Cyclin A availability is apparently the rate-limiting step for entry into mitosis, and cyclin A is required for completion of prophase (6).
Thymidine kinases play a critical role in generating the DNA synthetic precursor deoxythymidine triphosphate (dTTP). Cytoplasmic thymidine kinase 1 (TK1) expression and activity are regulated in a cell cycle-dependent manner, accumulating during G1-phase to peak levels in S-phase before being degraded prior to cell division (7).
The initiation of S phase begins with the formation of the pre-replication complex (pre-RC) in late mitosis/early G1 phase. CDT1 and cdc6 bind to the origin of DNA replication, which allows binding of the MCM2-7 complex. In order to ensure that replication occurs only once per cell cycle, geminin inhibits and destabilizes CDT1 during the S, G2 and M phases. At the metaphase/anaphase transition, geminin is degraded by the anaphase-promoting complex (APC) allowing for the formation of new pre-RC (8).

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

U.S. Patent No. 5,675,063.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

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Western blot analysis of extracts from various cell lines using Cyclin B1 (D5C10) XP® Rabbit mAb.
undefined Image 1: Cell Cycle Phase Determination Antibody Sampler Kit
Western blot analysis of extracts from various cell lines using Cyclin E1 (D7T3U) Rabbit mAb.
undefined Image 2: Cell Cycle Phase Determination Antibody Sampler Kit
Western blot analysis of extracts from HT-29 cells, mock treated (-) or aphidicolin-treated (10 μg/ml, 24 hr; +) and HCT 116 cells, wild-type (-) or thymidine kinase 1 knockout (+), using Thymidine Kinase 1 (E2H7Z) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).
undefined Image 3: Cell Cycle Phase Determination Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

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Immunohistochemical analysis of paraffin-embedded human ovarian papillary serous carcinoma using Thymidine Kinase 1 (E2H7Z) Rabbit mAb.
undefined Image 4: Cell Cycle Phase Determination Antibody Sampler Kit
Western blot analysis of extracts from C6 cells, untreated (-) or treated with Nocodazole (0.1 μg/ml, 18 hr; +), using Phospho-cdc2 (Tyr15) (10A11) Rabbit mAb (upper) or cdc2 Antibody #77055 (lower).
undefined Image 5: Cell Cycle Phase Determination Antibody Sampler Kit
Western bot analysis of extracts from various cell lines using Geminin (E5Q9S) XP® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).
undefined Image 6: Cell Cycle Phase Determination Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

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Western blot analysis of extracts from HeLa cells, either untreated or treated with Nocodazole #2190 (100 ng/ml, 16 hr), using Phospho-Histone H3 (Ser10) (D7N8E) XP® Rabbit mAb (upper) or Histone H3 (D1H2) Rabbit mAb #4499 (lower). Phospho-specificity of the antibody is shown by further treatment of the lysate with λ phosphatase.
undefined Image 7: Cell Cycle Phase Determination Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma using Phospho-Histone H3 (Ser10) (D7N8E) XP® Rabbit mAb performed on the Leica BOND RX.
undefined Image 8: Cell Cycle Phase Determination Antibody Sampler Kit
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.
undefined Image 9: Cell Cycle Phase Determination Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Western blot analysis of extracts from various cell lines using CDT1 (D10F11) Rabbit mAb.
undefined Image 10: Cell Cycle Phase Determination Antibody Sampler Kit
Western blot analysis of extracts from various cell lines using Cyclin A2 (E1D9T) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). In lane 6, HT-29 cells were treated with aphidicolin (10 µg/mL, 24 hr) to enrich cells at G1/S phase.
undefined Image 11: Cell Cycle Phase Determination Antibody Sampler Kit
Western blot analysis of extracts from HT-29 cells, synchronized in S-phase by double thymidine block (2 nM, 16 hr) followed by release into fresh media for the indicated time, using Cyclin B1 (D5C10) XP® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).
undefined Image 12: Cell Cycle Phase Determination Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Western blot analysis of extracts from HT-29 cells, untreated (-) or treated with aphidicolin (10 μg/ml; 24 hr; +) using Cyclin E1 (D7T3U) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).
undefined Image 13: Cell Cycle Phase Determination Antibody Sampler Kit
Immunoprecipitation of Thymidine Kinase 1 from HT-29 cell extracts treated with aphidicolin (10 μg/ml, 24 hr). Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is Thymidine Kinase 1 (E2H7Z) Rabbit mAb. Western blot analysis was performed using Thymidine Kinase 1 (E2H7Z) Rabbit mAb. Anti-rabbit IgG, HRP-linked Antibody #7074 was used as a secondary antibody.
undefined Image 14: Cell Cycle Phase Determination Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded human gastric adenocarcinoma using Thymidine Kinase 1 (E2H7Z) Rabbit mAb.
undefined Image 15: Cell Cycle Phase Determination Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Western blot analysis of extracts from HeLa cells, untreated or hydroxyurea treated for 20 hours, using Phospho-cdc2 (Tyr15) (10A11) Rabbit mAb.
undefined Image 16: Cell Cycle Phase Determination Antibody Sampler Kit
Immunoprecipitation of Geminin from 293T cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is Geminin (E5Q9S) XP® Rabbit mAb. Western blot analysis was performed using

Geminin (E5Q9S) XP® Rabbit mAb. Anti-rabbit IgG, HRP-linked Antibody #7074 was used as the secondary antibody.

undefined Image 17: Cell Cycle Phase Determination Antibody Sampler Kit
Confocal immunofluorescent analysis of mitotic HeLa cells using Phospho-Histone H3 (Ser10) (D7N8E) XP® Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
undefined Image 18: Cell Cycle Phase Determination Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

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Immunohistochemical analysis of paraffin-embedded human B-cell non-Hodgkin lymphoma using Phospho-Histone H3 (Ser10) (D7N8E) XP® Rabbit mAb performed on the Leica BOND RX.
undefined Image 19: Cell Cycle Phase Determination Antibody Sampler Kit
Immunoprecipitation of CDT1 from HeLa cells using CDT1 (D10F11) Rabbit mAb. Western blot was performed using the same antibody. Lane 1 is 10% input.
undefined Image 20: Cell Cycle Phase Determination Antibody Sampler Kit
Confocal immunofluorescent analysis of HT-29 cells using Cyclin B1 (D5C10) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
undefined Image 21: Cell Cycle Phase Determination Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Confocal immunofluorescent analysis of HCT 116 cells, wild-type (left, positive) or thymidine kinase 1 knockout (right, negative), using Thymidine Kinase 1 (E2H7Z) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
undefined Image 22: Cell Cycle Phase Determination Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded human endometrioid adenocarcinoma using Thymidine Kinase 1 (E2H7Z) Rabbit mAb.
undefined Image 23: Cell Cycle Phase Determination Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded 293T cell pellet (left, high-expressing) or SK-MEL-28 cell pellet (right, low-expressing) using Geminin (E5Q9S) XP® Rabbit mAb.
undefined Image 24: Cell Cycle Phase Determination Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

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Flow cytometric analysis of Jurkat cells using Phospho-Histone H3 (Ser10) (D7N8E) XP® Rabbit mAb and Propidium Iodide (PI)/RNase Staining Solution #4087 to measure DNA content. Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.
undefined Image 25: Cell Cycle Phase Determination Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma using Phospho-Histone H3 (Ser10) (D7N8E) XP® Rabbit mAb.
undefined Image 26: Cell Cycle Phase Determination Antibody Sampler Kit
Confocal immunofluorescent analysis of HT-29 and HUVEC cells using CDT1 (D10F11) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
undefined Image 27: Cell Cycle Phase Determination Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Flow cytometric analysis of HCT 116 cells, wild-type (green, positive) or thymidine kinase 1 knockout (blue, negative), using Thymidine Kinase 1 (E2H7Z) Rabbit mAb (solid lines) or a concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.
undefined Image 28: Cell Cycle Phase Determination Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded human B-cell non-Hodgkin lymphoma using Thymidine Kinase 1 (E2H7Z) Rabbit mAb.
undefined Image 29: Cell Cycle Phase Determination Antibody Sampler Kit
Confocal immunofluorescent analysis of asynchronous HeLa cells labeled with Phospho-cdc2 (Tyr15) (10A11) Rabbit mAb (green) and Phospho-Histone H3 (Ser10) (6G3) Mouse mAb #9706 (red).
undefined Image 30: Cell Cycle Phase Determination Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

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Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma using Geminin (E5Q9S) XP® Rabbit mAb.
undefined Image 31: Cell Cycle Phase Determination Antibody Sampler Kit
HeLa cells were cultured and either left untreated (left panel) or treated with Nocodazole #2190 (100 ng/ml, 16 hr; right panel). Chromatin immunoprecipitations were performed with cross-linked chromatin and Phospho-Histone H3 (Ser10) (D7N8E) XP® Rabbit mAb or Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human GAPDH Promoter Primers #4471 and SimpleChIP® Human γ-Actin Promoter Primers #5037. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
undefined Image 32: Cell Cycle Phase Determination Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded human endometrioid adenocarcinoma using Phospho-Histone H3 (Ser10) (D7N8E) XP® Rabbit mAb.
undefined Image 33: Cell Cycle Phase Determination Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

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Flow cytometric analysis of Jurkat cells using Cyclin B1 (D5C10) XP® Rabbit mAb (right) and Propidium Iodide (PI)/RNase Staining Solution #4087, compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (left). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.
undefined Image 34: Cell Cycle Phase Determination Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded human squamous cell lung carcinoma using Thymidine Kinase 1 (E2H7Z) Rabbit mAb.
undefined Image 35: Cell Cycle Phase Determination Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded human esophageal carcinoma using Geminin (E5Q9S) XP® Rabbit mAb.
undefined Image 36: Cell Cycle Phase Determination Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

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Immunohistochemical analysis of paraffin-embedded human prostate adenocarcinoma using Phospho-Histone H3 (Ser10) (D7N8E) XP® Rabbit mAb.
undefined Image 37: Cell Cycle Phase Determination Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma using Thymidine Kinase 1 (E2H7Z) Rabbit mAb.
undefined Image 38: Cell Cycle Phase Determination Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded human endometrioid adenocarcinoma using Geminin (E5Q9S) XP® Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right).
undefined Image 39: Cell Cycle Phase Determination Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

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Immunohistochemical analysis of paraffin-embedded human infiltrating ductal carcinoma of the breast using Phospho-Histone H3 (Ser10) (D7N8E) XP® Rabbit mAb.
undefined Image 40: Cell Cycle Phase Determination Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded human tonsil using Thymidine Kinase 1 (E2H7Z) Rabbit mAb.
undefined Image 41: Cell Cycle Phase Determination Antibody Sampler Kit
Flow cytometric analysis of Jurkat cells using Phospho-cdc2 (Tyr15) (10A11) Rabbit mAb (right) or concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (left), co-stained with Propidium Iodide (PI)/RNase Staining Solution #4087. Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.
undefined Image 42: Cell Cycle Phase Determination Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma using Geminin (E5Q9S) XP® Rabbit mAb.
undefined Image 43: Cell Cycle Phase Determination Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded normal human thymus using Phospho-Histone H3 (Ser10) (D7N8E) XP®
undefined Image 44: Cell Cycle Phase Determination Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded normal human small intestine using Thymidine Kinase 1 (E2H7Z) Rabbit mAb.
undefined Image 45: Cell Cycle Phase Determination Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Immunohistochemical analysis of paraffin-embedded human B-cell non-Hodgkin's lymphoma using Geminin (E5Q9S) XP® Rabbit mAb.
undefined Image 46: Cell Cycle Phase Determination Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded normal human esophagus using Phospho-Histone H3 (Ser10) (D7N8E) XP® Rabbit mAb.
undefined Image 47: Cell Cycle Phase Determination Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded ductal breast carcinoma using Thymidine Kinase 1 (E2H7Z) Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right).
undefined Image 48: Cell Cycle Phase Determination Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

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Immunohistochemical analysis of paraffin-embedded human ovarian serous carcinoma using Geminin (E5Q9S) XP® Rabbit mAb.
undefined Image 49: Cell Cycle Phase Determination Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded human tonsil using Phospho-Histone H3 (Ser10) (D7N8E) XP® Rabbit mAb.
undefined Image 50: Cell Cycle Phase Determination Antibody Sampler Kit
Confocal immunofluorescent analysis of Hep G2 cells (left) and SK-MEL-28 cells (right) using Geminin (E5Q9S) XP® Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
undefined Image 51: Cell Cycle Phase Determination Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

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Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma, untreated (left) or treated with Lambda Protein Phosphatase Kit #89726 (right) using Phospho-Histone H3 (Ser10) (D7N8E) XP® Rabbit mAb.
undefined Image 52: Cell Cycle Phase Determination Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded CT26.WT syngeneic tumor using Phospho-Histone H3 (Ser10) (D7N8E) XP® Rabbit mAb.
undefined Image 53: Cell Cycle Phase Determination Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded HT-29 cell pellet, untreated (left, negative) or treated with nocodazole, 100 ng/mL, 24 hr, (right, positive) using Phospho-Histone H3 (Ser10) (D7N8E) XP® Rabbit mAb.
undefined Image 54: Cell Cycle Phase Determination Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

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Immunohistochemical analysis of paraffin-embedded mouse thymus using Phospho-Histone H3 (Ser10) (D7N8E) XP® Rabbit mAb.
undefined Image 55: Cell Cycle Phase Determination Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded mouse spleen using Phospho-Histone H3 (Ser10) (D7N8E) XP® Rabbit mAb.
undefined Image 56: Cell Cycle Phase Determination Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma using Phospho-Histone H3 (Ser10) (D7N8E) XP® Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right).
undefined Image 57: Cell Cycle Phase Determination Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

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Simple Western™ analysis of lysates (0.1 mg/mL) from HeLa cells using Cyclin B1 (D5C10) XP® Rabbit mAb #12231. The virtual lane view (left) shows the target band (as indicated) at 1:10 and 1:50 dilutions of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at 1:10 (blue line) and 1:50 (green line) dilutions of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a BioTechne brand, using the 12-230 kDa separation module.
undefined Image 58: Cell Cycle Phase Determination Antibody Sampler Kit
Immunoprecipitation of Cyclin B1 protein from HeLa cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is Cyclin B1 (D5C10) XP® Rabbit mAb. Western blot analysis was performed using Cyclin B1 (D5C10) XP® Rabbit mAb. Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb #3678 was used as a secondary antibody.
undefined Image 59: Cell Cycle Phase Determination Antibody Sampler Kit
Simple WesternTM analysis of lysates (0.1 mg/mL) from Jurkat cells using Cyclin A2 (E1D9T) Rabbit mAb #91500. The virtual lane view (left) shows the target band (as indicated) at 1:10 and 1:50 dilutions of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at 1:10 (blue line) and 1:50 (green line) dilutions of primary antibody. This experiment was performed under reducing conditions on the JessTM Simple Western instrument from ProteinSimple, a BioTechne brand, using the 12-230 kDa separation module.
undefined Image 60: Cell Cycle Phase Determination Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.