Cell Cycle Phase Determination Antibody Sampler Kit #17498
Product Information
Kit Usage Information
Protocols
- 4539: Western Blotting, Immunoprecipitation (Agarose), Immunofluorescence, Flow
- 7074: Western Blotting
- 8064: Western Blotting, Immunoprecipitation (Agarose), Immunofluorescence
- 12231: Western Blotting, Immunoprecipitation (Magnetic), Immunofluorescence, Flow
- 20808: Western Blotting
- 28755: Western Blotting, Immunoprecipitation (Agarose), Immunohistochemistry (Paraffin), Immunofluorescence, Flow
- 52508: Western Blotting, Immunoprecipitation (Agarose), Immunohistochemistry (Paraffin), Immunofluorescence
- 53348: Western Blotting, Immunoprecipitation (Agarose), Immunohistochemistry (Leica® Bond™), Immunohistochemistry (Paraffin), Immunofluorescence, Flow, ChIP Magnetic
- 91500: Western Blotting
Product Description
Specificity / Sensitivity
Cyclin A2 (E1D9T) Rabbit mAb recognizes endogenous levels of total cyclin A2 protein.
Cyclin B1 (D5C10) XP® Rabbit mAb recognizes endogenous levels of total cyclin B1 protein. This antibody also detects a 100 kDa protein of unknown origin in some cell lines.
Cyclin E1 (D7T3U) Rabbit mAb recognizes endogenous levels of total cyclin E1 protein. Based on the sequence of the peptide antigen, this antibody is expected to detect all isoforms of cyclin E1. This antibody does not cross-react with cyclin E2.
Phospho-cdc2 (Tyr15) (10A11) Rabbit mAb detects endogenous levels of cdc2 protein only when phosphorylated at tyrosine 15.
Geminin (E5Q9S) XP® Rabbit mAb recognizes endogenous levels of total geminin protein.
CDT1 (D10F11) Rabbit mAb recognizes endogenous levels of total CDT1 protein.
Thymidine Kinase 1 (E2H7Z) Rabbit mAb recognizes endogenous levels of total thymidine kinase 1 protein.
Source / Purification
Background
Phosphorylation of Histone H3 at Ser10 is tightly correlated with chromosome condensation during both mitosis and meiosis (4).
Overcoming the G1/S checkpoint to commence DNA replication requires cyclin E, traversing the G2/M checkpoint to initiate mitosis requires cyclin B, and cyclin A is required for both S-phase and M-phase (5). Cyclin A availability is apparently the rate-limiting step for entry into mitosis, and cyclin A is required for completion of prophase (6).
Thymidine kinases play a critical role in generating the DNA synthetic precursor deoxythymidine triphosphate (dTTP). Cytoplasmic thymidine kinase 1 (TK1) expression and activity are regulated in a cell cycle-dependent manner, accumulating during G1-phase to peak levels in S-phase before being degraded prior to cell division (7).
The initiation of S phase begins with the formation of the pre-replication complex (pre-RC) in late mitosis/early G1 phase. CDT1 and cdc6 bind to the origin of DNA replication, which allows binding of the MCM2-7 complex. In order to ensure that replication occurs only once per cell cycle, geminin inhibits and destabilizes CDT1 during the S, G2 and M phases. At the metaphase/anaphase transition, geminin is degraded by the anaphase-promoting complex (APC) allowing for the formation of new pre-RC (8).
- Atherton-Fessler, S. et al. (1994) Mol Biol Cell 5, 989-1001.
- Gong, D. and Ferrell, J.E. (2010) Mol Biol Cell 21, 3149-61.
- Norbury, C. et al. (1991) EMBO J 10, 3321-9.
- Hendzel, M.J. et al. (1997) Chromosoma 106, 348-60.
- Pagano, M. et al. (1992) EMBO J 11, 961-71.
- Furuno, N. et al. (1999) J Cell Biol 147, 295-306.
- Munch-Petersen, B. (2010) Nucleosides Nucleotides Nucleic Acids 29, 363-9.
- Caillat, C. and Perrakis, A. (2012) Subcell Biochem 62, 71-87.
Limited Uses
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