WB, IP
H
Endogenous
110
Rabbit IgG
#Q86WJ1
9557
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly231 of human CHD1L protein.
Background
Chromodomain-helicase-DNA-binding domain 1 like (CHD1L) is a DNA helicase and member of the SNF2 subfamily of ATP dependent chromatin remodeling enzymes (1). Unlike other CHD chromatin remodeling proteins, CHD1L lacks a methylated histone binding chromodomain but does contain a macro domain that binds poly-ADP ribosylated (PARylated) targets (1,2). Following genotoxic stress, CHD1L interacts with PARylated PARP1 and is recruited to sites of DNA damage to facilitate DNA repair (3,4). The CHD1L protein is often over expressed in metastatic hepatocellular carcinoma (HCC) and the corresponding CHD1L gene is located in a region that is frequently amplified in cases of HCC (5-7). Research studies indicate that CHD1L over expression may lead to over relaxation of chromatin and exposing the underlying DNA to genotoxic stress (1). CHD1L can regulate expression of genes that promote tumor cell proliferation, migration and metastasis, providing another mechanism where CHD1L may promote hepatocellular carcinoma progression and metastasis (6,8). Additional research studies suggest that over expression of CHD1L may be involved in the progression of bladder, colon and ovary cancer (9-11).
- Deng, W. (2009) Cell Stem Cell 5, 349-50.
- Karras, G.I. et al. (2005) EMBO J 24, 1911-20.
- Gottschalk, A.J. et al. (2009) Proc Natl Acad Sci U S A 106, 13770-4.
- Ahel, D. et al. (2009) Science 325, 1240-3.
- Ma, N.F. et al. (2008) Hepatology 47, 503-10.
- Chen, L. et al. (2010) J Clin Invest 120, 1178-91.
- Chen, L. et al. (2009) Hepatology 50, 122-9.
- Chen, M. et al. (2009) PLoS One 4, e6727.
- He, W.P. et al. (2012) BMC Cancer 12, 437.
- Ji, X. et al. (2013) J Surg Res 185, 84-91.
- Tian, F. et al. (2013) Tumour Biol , [Epub ahead of print].
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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