WB, IP
H M R
Endogenous
28
Rabbit IgG
#Q9UQN3
25978
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu171 of human CHMP2B protein.
Background
CHMP2B is a component of the ESCRT III (endosomal sorting required for transport complex III) complex (1, 2). The ESCRT system is composed of the ESCRT-0, -I, -II, and -III complexes, which function sequentially to direct the transport of ubiquitinated transmembrane proteins into the intralumenal vesicles (ILVs), which will eventually mature into multivesicular bodies (MVBs). CHMP2B is a homolog of yeast Vps2, which functions in the ESCRT-II complex to change the initial spiral-structure of snf7 into membrane-sculpting helices for the final pinch off process (3). CHMP2B probably functions similarly in mammalian cells. Research studies show that manipulation of the ESCRT-III complex leads to accumulation of CHMP2B at the plasma membrane and overexpressed CHMP2B polymerizes into a tight helical structure that deforms the shape of associated plasma membrane (4).
Research studies have shown that mutation of CHMP2B is associated with frontotemporal dementia, (5, 6). Studies have further shown that the dysfunction of mutant CHMP2B expression may disrupts the normal endo-autophagosome and endo-lysosome pathways and lead to neurodegenerative diseases (6-9).
- McCullough, J. et al. (2013) Annu Rev Biochem 82, 663-92.
- Henne, W.M. et al. (2013) Cold Spring Harb Perspect Biol 5, (9).
- Henne, W.M. et al. (2012) Cell 151, 356-71.
- Bodon, G. et al. (2011) J Biol Chem 286, 40276-86.
- Skibinski, G. et al. (2005) Nat Genet 37, 806-8.
- Lee, J.A. et al. (2007) Curr Biol 17, 1561-7.
- Filimonenko, M. et al. (2007) J Cell Biol 179, 485-500.
- Han, J.H. et al. (2012) Biochem Biophys Res Commun 421, 544-9.
- Urwin, H. et al. (2010) Hum Mol Genet 19, 2228-38.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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