WB, IP
H
Endogenous
28
Rabbit
#Q16740
8192
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro263 of human CLPP protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
The proteolytic component, Tetradecameric Peptidase (CLpP), is a hexamer in one of four ATP-dependent mitochondrial proteases (CLpXP). CLPP, one of the proteases, is an endopeptidase that is highly conserved among prokaryotes and eukaryotes, both at the level of amino acid sequence and quaternary structure. The active unit of CLPP is a barrel-shaped tetradecamer, Proteolysis of larger substrates is initiated by caseinolytic peptidase X (CLPX) which unfolds specific protein substrates. The unfolded polypeptide chain translocates into the CLPP proteolytic chamber for protein degradation within the interior chamber of mitochondria (1). Recessive mutations in CLPP cause Perrault Syndrome, a heterogeneous condition characterized by sensorineural hearing loss and ovarian failure (2).
Mutations in Parkin or PINK1 cause recessively inherited Parkinson’s disease. In healthy mitochondria, PINK1 is rapidly degraded by mitochondrial proteases and the proteasome. Upon mitochondrial depolarization, PINK1 accumulates on the mitochondrial surface, recruits Parkin from the cytosol, and initiates mitophagy. The mitochondrial proteases MPP, PARL, m-AAA and CLPP have been implicated in PINK1 degradation and cleavage (3).
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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