WB, IP
H M
Endogenous
22
Rabbit
#Q8IZR5
146223
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:200 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse
Species predicted to react based on 100% sequence homology
The antigen sequence used to produce this antibody shares
100% sequence homology with the species listed here, but
reactivity has not been tested or confirmed to work by CST.
Use of this product with these species is not covered under
our
Product Performance Guarantee.
Hamster, Bovine
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu202 of human CMTM4 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
CKLF-like MARVEL transmembrane domain-containing protein 4 (CMTM4) is a member of the chemokine-like factor (CKLF)-like MARVEL transmembrane domain-containing family (1). CMTM4 acts as a tumor suppressor in various malignancies, and regulates cell growth and transition through the cell cycle in HeLa cells (1-4). CMTM4 plays an important role in angiogenesis, enabling internalization of membrane-bound vascular endothelial cadherin at adherens junctions, mediating endothelial barrier function, and controlling vascular sprouting (5). In the immune system, CMTM4 acts as a backup for CMTM6 to regulate plasma membrane expression of PD-L1, an immune inhibitory ligand critical for immune tolerance to self and anti-tumor immunity (6-8). CMTM4 may also protect PD-L1 from being polyubiquitinated and targeted for degradation (8). Due to the roles of CMTM4 in the immune system and as a tumor suppressor, it is being investigated as a therapeutic target for the treatment of cancer.
- Plate, M. et al. (2010) Mol Cells 29, 355-61.
- Li, T. et al. (2015) J Exp Clin Cancer Res 34, 122.
- Zhang, H. et al. (2015) Iran J Basic Med Sci 18, 1020-6.
- Bei, C. et al. (2017) Onco Targets Ther 10, 5439-43.
- Chrifi, I. et al. (2018) Angiogenesis , .
- Mezzadra, R. et al. (2017) Nature 549, 106-10.
- Imamovic, D. and Vranic, S. (2017) Ann Transl Med 5, 467.
- Burr, M.L. et al. (2017) Nature 549, 101-5.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
Limited Uses
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