WB, IP
H M R
Endogenous
26-34
Rabbit
#O15431
1317
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro107 of human CTR1 (SLC31A1) protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
The high affinity copper uptake protein 1 (CTR1, SLC31A1) helps maintain copper homeostasis by mediating dietary copper intake chiefly in the small intestine (1). A series of methionine-rich repeats and other residues are conserved among CTR1 genes across taxa, and are thought to be important for copper transport (2,3). In mammalian cells, CTR1 is localized to the plasma membrane and intracellular vesicles (3). Upon copper uptake via plasma membrane into cells, CTR1 is down regulated by clathrin-dependent endocytosis and degradation of CTR1 protein (4). Research studies suggest that the CTR1 copper transporter also mediates uptake of the anticancer drug cisplatin in yeast and mammals and that decreased CTR1 can result in the development of cisplatin resistance (5,6). Treatment of cancer cells with cisplatin can result in reduced CTR1 expression, which reduces cisplatin accumulation within cells and leads to cisplatin resistance in some human cancer cells (7-9).
- Lee, J. et al. (2001) Proc Natl Acad Sci U S A 98, 6842-7.
- Kim, B.E. et al. (2008) Nat Chem Biol 4, 176-85.
- Lee, J. et al. (2002) J Biol Chem 277, 4380-7.
- Petris, M.J. et al. (2003) J Biol Chem 278, 9639-46.
- Ishida, S. et al. (2002) Proc Natl Acad Sci U S A 99, 14298-302.
- Kuo, M.T. et al. (2007) Cancer Metastasis Rev 26, 71-83.
- Abada, P. and Howell, S.B. (2010) Met Based Drugs 2010, 317581.
- Yu, L. et al. (2011) Nan Fang Yi Ke Da Xue Xue Bao 31, 801-4.
- Kalayda, G.V. et al. (2012) J Inorg Biochem 116, 1-10.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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