Revision 4

#29603Store at -20C

1 Kit

(7 x 20 microliters)

Cell Signaling Technology

Orders: 877-616-CELL (2355) [email protected]

Support: 877-678-TECH (8324)

Web: [email protected] cellsignal.com

3 Trask LaneDanversMassachusetts01923USA
For Research Use Only. Not for Use in Diagnostic Procedures.
Product Includes Product # Quantity Mol. Wt Isotype/Source
Fas (C18C12) Rabbit mAb 4233 20 µl 40-50 kDa Rabbit IgG
TNF-R1 (C25C1) Rabbit mAb 3736 20 µl 55 kDa Rabbit IgG
TNF-R2 (E8D7P) Rabbit mAb 72337 20 µl 60-80 kDa Rabbit IgG
DR3 (D4O3X) Rabbit mAb 20772 20 µl 55-60 kDa Rabbit IgG
DR4 (D9S1R) Rabbit mAb 42533 20 µl 35-55 kDa Rabbit IgG
DR5 (D4E9) XP® Rabbit mAb 8074 20 µl 40, 48 kDa Rabbit IgG
DR6 (E8D2I) Rabbit mAb 93026 20 µl 80, 120 kDa Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl Goat 

Please visit cellsignal.com for individual component applications, species cross-reactivity, dilutions, protocols, and additional product information.

Description

The Death Receptor Antibody Sampler Kit II provides an economical means to investigate members of the death receptor family. The kit includes enough antibody to perform two western blot experiments with each primary antibody.

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Background

The tumor necrosis factor receptor family, which includes TNF-RI, TNF-R2, Fas, DR3, DR4, DR5, and DR6, plays an important role in the regulation of apoptosis in various physiological systems (1,2). The receptors are activated by a family of cytokines that include TNF, FasL, TWEAK, and TRAIL. They are characterized by a highly conserved extracellular region containing cysteine-rich repeats and a conserved intracellular region of about 80 amino acids termed the death domain (DD). The DD is important for transducing the death signal by recruiting other DD containing adaptor proteins (FADD, TRADD, RIP) to the death-inducing signaling complex (DISC) resulting in activation of caspases. The two receptors for TNF-α, TNF-R1 (55 kDa) and TNF-R2 (75 kDa) can mediate distinct cellular responses (3,4). In most cases cytotoxicity elicited by TNF has been reported to act through TNF-R1 (5,6). DR3/WSL-1/Apo-3/TRAMP/LARD is a TNFR family member containing the characteristic extracellular cysteine-repeats, transmembrane region, and an intracellular DD (7-11). DR3 is activated by its ligand Apo-3L/TWEAK to induce apoptosis and activation of NF-κB (12,13). Like TNF-R1, DR3 binds to the DD adaptor protein TRADD, which can then associate with other DD proteins like FADD and RIP as well as members of the TRAF family (7,8). Tissue expression of DR3 is very restricted, primarily seen on the surface of activated thymocytes and lymphocytes and plays an important role in thymocyte negative selection (7,8,14). Studies have also indicated an association with DR3 and rheumatoid arthritis (15,16). DR4 (TRAIL-RI, TNFRSF10A) and DR5 (TRAIL-R2, TNFRSF10B) are receptors for the cytokine TRAIL. Both receptors contain death domains that recruit DISC complexes triggering caspase activation and apoptosis (17-20). DR6, also known as TNFRSF21, is a TNFR family member able to induce apoptosis as well as activation of NF-κB and JNK (21). DR6 appears to play a critical role in the activation and differentiation of T and B lymphocytes (22,23). In the nervous system, β-amyloid precursor protein (APP) activates DR6 to trigger neuronal degeneration (24).

  1. Nagata, S. (1997) Cell 88, 355-65.
  2. Thorburn, A. (2004) Cell Signal 16, 139-44.
  3. Tartaglia, L.A. et al. (1991) Proc Natl Acad Sci U S A 88, 9292-6.
  4. Peschon, J.J. et al. (1998) J Immunol 160, 943-52.
  5. Tartaglia, L.A. et al. (1993) Cell 73, 213-6.
  6. Rothe, J. et al. (1993) Nature 364, 798-802.
  7. Chinnaiyan, A.M. et al. (1996) Science 274, 990-2.
  8. Kitson, J. et al. (1996) Nature 384, 372-5.
  9. Marsters, S.A. et al. (1996) Curr Biol 6, 1669-76.
  10. Bodmer, J.L. et al. (1997) Immunity 6, 79-88.
  11. Screaton, G.R. et al. (1997) Proc Natl Acad Sci U S A 94, 4615-9.
  12. Marsters, S.A. et al. (1998) Curr Biol 8, 525-8.
  13. Kaptein, A. et al. (2000) FEBS Lett 485, 135-41.
  14. Wang, E.C. et al. (2001) Mol Cell Biol 21, 3451-61.
  15. Osawa, K. et al. (2004) Genes Immun 5, 439-43.
  16. Borysenko, C.W. et al. (2005) Biochem Biophys Res Commun 328, 794-9.
  17. Pan, G. et al. (1997) Science 276, 111-3.
  18. Walczak, H. et al. (1997) EMBO J 16, 5386-97.
  19. Chaudhary, P.M. et al. (1997) Immunity 7, 821-30.
  20. Schneider, P. et al. (1997) Immunity 7, 831-6.
  21. Pan, G. et al. (1998) FEBS Lett 431, 351-6.
  22. Zhao, H. et al. (2001) J Exp Med 194, 1441-8.
  23. Schmidt, C.S. et al. (2003) J Exp Med 197, 51-62.
  24. Nikolaev, A. et al. (2009) Nature 457, 981-9.

Background References

    Trademarks and Patents

    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    XP is a registered trademark of Cell Signaling Technology, Inc.
    U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.
    All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

    Limited Uses

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    Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

    Orders: 877-616-CELL (2355)•[email protected]• Support: 877-678-TECH (8324)•[email protected]• Web: cellsignal.com
    For Research Use Only. Not for Use in Diagnostic Procedures.