WB
All
Transfected Only
Rabbit IgG
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:10000 |
Storage
Specificity / Sensitivity
Species Reactivity:
All Species Expected
Source / Purification
Monoclonal antibody is produced by immunizing animals with KLH-conjugated dinitrophenol.
Background
The synthetic compound dinitrophenol (DNP) is a small hapten molecule that can be conjugated to proteins for use in immunoassays (1). Antibodies that recognize dinitrophenol will display strong binding affinity to DNP conjugated molecules, including nucleic acids and proteins. These DNP-conjugated compounds can be used in in situ hybridization, western, northern, and Southern blotting (1,2). Dinitrophenol is a commonly used insecticide and has been used as a treatment for weight loss (3).
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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