WB
H M R Mk
Endogenous
120
Rabbit
#Q9Y2L1
22894
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val928 of human DIS3 protein. Antibodies are purified by peptide affinity chromatography.
Background
DIS3, also known as RRP44, is a conserved protein responsible for the core catalytic activities of the exosome complex, which degrades RNA (1-3). With two catalytic sites, DIS3 possesses both endoribonucleolytic and 3’-to-5’ exoribonucleolytic activity (2,4). It plays very diverse roles in RNA metabolism, including control of aberrant pre-mRNA turnover, gene expression, and RNA processing (5-9). DIS3 also plays a role in mitotic progression by helping form kinetochores (9,10). Additionally, DIS3 is implicated in antibody diversity by helping the exosome target activation-induced cytidine deaminase to the DNA strands in B cells. DIS3 and other exosome components are upregulated during V(D)J recombination and B cells in knockout mice are unable to develop past the pro-B cell stage (11,12). Mutations in the DIS3 gene have been well described in multiple myeloma (13,14).
- Vincent, H.A. and Deutscher, M.P. (2006) J Biol Chem 281, 29769-75.
- Dziembowski, A. et al. (2007) Nat Struct Mol Biol 14, 15-22.
- Liu, Q. et al. (2006) Cell 127, 1223-37.
- Lebreton, A. et al. (2008) Nature 456, 993-6.
- Bousquet-Antonelli, C. et al. (2000) Cell 102, 765-75.
- Milligan, L. et al. (2005) Mol Cell Biol 25, 9996-10004.
- Mukherjee, D. et al. (2002) EMBO J 21, 165-74.
- Allmang, C. et al. (1999) EMBO J 18, 5399-410.
- Murakami, H. et al. (2007) PLoS One 2, e317.
- Kinoshita, N. et al. (1991) Mol Cell Biol 11, 5839-47.
- Basu, U. et al. (2011) Cell 144, 353-63.
- Laffleur, B. et al. (2022) Sci Immunol 7, eabn2738.
- Chapman, M.A. et al. (2011) Nature 471, 467-72.
- Walker, B.A. et al. (2012) Blood 120, 1077-86.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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