WB, IP, IF-IC
H
Endogenous
105
Rabbit IgG
#A0AVK6
79733
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| Immunofluorescence (Immunocytochemistry) | 1:800 - 1:3200 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human E2F8 protein.
Background
The E2F family consists of eight transcription factors that regulate gene expression to control cell cycle progression. The atypical E2F family members, E2F-7 and E2F-8, are unique in that they have two distinct DNA-binding domains, but lack the DP-heterodimerization, retinoblastoma-binding, and transcriptional activation domains contained in the canonical E2F family members. E2F-7 and E2F-8 are thought to function primarily as transcriptional repressors (1).
E2F8 is highly expressed in multiple human cancers, including hepatocellular carcinoma, lung, breast, ovarian and cervical cancer, and may serve as a prognostic marker (1-5). In cancer cell lines, E2F8 expression enhances proliferation, migration, invasion, and the expression of epithelial mesenchymal transition (EMT) markers (2,3).
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation IF-IC: Immunofluorescence (Immunocytochemistry)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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