| Product Includes | Product # | Quantity | Mol. Wt | Isotype/Source |
|---|---|---|---|---|
| MMP-1 (E9S9N) Rabbit mAb | 54376 | 20 µl | 55 kDa | Rabbit IgG |
| MMP-2 (D4M2N) Rabbit mAb | 40994 | 20 µl | 64,72 kDa | Rabbit IgG |
| MMP-9 (D6O3H) XP® Rabbit mAb | 13667 | 20 µl | 84, 92 kDa | Rabbit IgG |
| MMP-13 (E4W3T) Rabbit mAb | 69926 | 20 µl | 60 kDa | Rabbit IgG |
| Fibronectin/FN1 (E5H6X) Rabbit mAb | 26836 | 20 µl | 300 kDa | Rabbit IgG |
| MMP-3 (D7F5B) Rabbit mAb | 14351 | 20 µl | 60 kDa | Rabbit IgG |
| LOX (D8F2K) Rabbit mAb | 58135 | 20 µl | 54, 56 kDa | Rabbit IgG |
| COL11A1 (E6O7R) Rabbit mAb | 70458 | 20 µl | 250 kDa | Rabbit IgG |
| COL1A1 (E6A8E) Rabbit mAb | 39952 | 20 µl | 220 kDa | Rabbit IgG |
| Anti-rabbit IgG, HRP-linked Antibody | 7074 | 100 µl | Goat |
Please visit cellsignal.com for individual component applications, species cross-reactivity, dilutions, protocols, and additional product information.
Description
The ECM Profiling Antibody Sampler Kit provides an economical means of detecting endogenous levels of the specific ECM components using the corresponding antibodies. The kit includes enough antibodies to perform two western blot experiments with each primary antibody.
Storage
Background
The extracellular matrix (ECM) is a cell surface associated three-dimensional macromolecular network composed of collagens, proteoglycans/glycosaminoglycans, elastin, fibronectin, laminins, and several other glycoproteins (1). The network provides a dynamic microenvironment surrounding the cell enabling it to carry on its function. Among the ECM proteins, fibronectin functions as a mediator to bridge distinct ECM components such as collagens, growth factors, as well as cell surface integrins to regulate ECM structural change and initiate signaling pathways (2). During normal development and pathological conditions, the ECM network is highly dynamic and continuously undergoes remodeling marked by the change of the ECM structural components, such as COL1A1, COL11A1, fibronectin, and versican. The matrix-degrading enzyme MMPs such as MMP1, MMP2, and MMP9 are highly involved in this process (4). Additional players in this process are the LOX family members of lysyl oxidase. They catalyze the first step of the covalent cross-linking of ECM proteins, collagens, and elastin, which contributes to ECM stiffness and mechanical properties (5).
- Theocharis, A.D. et al. (2019) FEBS J 286, 2830-69.
- Zollinger, A.J. and Smith, M.L. (2017) Matrix Biol 60-1, 27-37.
- Paolillo, M. and Schinelli, S. (2019) Int J Mol Sci 20, pii: E4947. doi: 10.3390/ijms20194947.
- Bonnans, C. et al. (2014) Nat Rev Mol Cell Biol 15, 786-801.
- Amendola, P.G. et al. (2019) Cancers (Basel) 11, pii: E729. doi: 10.3390/cancers11050729.
Background References
Trademarks and Patents
Limited Uses
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