| Products Included | No. | Volume | Applicaton | Dilution | Reactivity | Homology† |
|---|---|---|---|---|---|---|
| Primary Cocktail | 7972 | 100 µl | Immunofluorescence (Immunocytochemistry), Immunofluorescence (Paraffin)‡ | 1:100 | Human, Monkey | Mouse |
| Detection Cocktail | 7973 | 100 µl | Immunofluorescence (Immunocytochemistry), Immunofluorescence (Paraffin)‡ | 1:100 | N/A | N/A |
†Species predicted to react based on 100% sequence homology.
‡Immunofluorescence (Paraffin) protocol recommended unmasking buffer: EDTA
| Kit Analytes | Detection Dye | Ex(max) (nm) | Em(max) (nm) |
|---|---|---|---|
| EGF Receptor | Alexa Fluor® 555 | 555 | 565 |
| Phospho-EGF Receptor (Tyr1068) | Alexa Fluor® 488 | 495 | 519 |
| Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) | Alexa Fluor® 647 | 650 | 665 |
IF-P, IF-IC
#P27361, #P28482, #P00533
5595, 5594, 1956
Product Information
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Monkey
Source / Purification
Monoclonal antibodies were produced by immunizing animals with synthetic phosphopeptides corresponding to residues surrounding Thr202/Tyr204 of human p44 MAP kinase and Tyr1068 of human EGF receptor, or with a fusion protein containing the cytoplasmic domain of human EGF receptor.
Product Description
| MW (kDa) | n/a |
Background
The epidermal growth factor receptor (EGFR) is a 170 kDa transmembrane receptor tyrosine kinase that belongs to the HER/ErbB protein family that includes HER2/ErbB2/neu, HER3/ErbB3, and HER4/ErbB4 (1,2). Ligand binding results in receptor homo- and heterodimerization which stimulates its intrinsic tyrosine kinase activity. Autophosphorylation at residues Tyr992, Tyr1045, Tyr1068, Tyr1148, and Tyr1173, as well as c-Src mediated phosphorylation at Tyr845 and Tyr1101 promote docking of SH2 domain-bearing signaling proteins resulting in cellular responses to EGFR activation (2-6). For example, phosphorylation of Tyr1068 facilitates recruitment of Grb2 which, via association with Sos, stimulates the GTP binding activity of Ras, leading to the activation of MAP kinase and other signaling cascades (7). Following activation, EGFR is rapidly endocytosed and either recycled back to the plasma membrane or targeted for lysosomal degradation (8). Dysregulation of EGFR signaling through activating mutations or gene amplification has been implicated in the pathogenesis of many human malignancies, leading to intense clinical study of this pathway (9-13).
- Hackel, P.O. et al. (1999) Curr Opin Cell Biol 11, 184-9.
- Zwick, E. et al. (1999) Trends Pharmacol Sci 20, 408-12.
- Cooper, J.A. and Howell, B. (1993) Cell 73, 1051-4.
- Emlet, D.R. et al. (1997) J Biol Chem 272, 4079-86.
- Emlet, D.R. et al. (1997) J Biol Chem 272, 4079-86.
- Levkowitz, G. et al. (1999) Mol Cell 4, 1029-40.
- Rojas, M. et al. (1996) J Biol Chem 271, 27456-61.
- Lai, W.H. et al. (1989) J Cell Biol 109, 2741-9.
- Yarden, Y. and Sliwkowski, M.X. (2001) Nat Rev Mol Cell Biol 2, 127-37.
- Press, M.F. and Lenz, H.J. (2007) Drugs 67, 2045-75.
- Baselga, J. (2002) Oncologist 7 Suppl 4, 2-8.
- Foon, K.A. et al. (2004) Int J Radiat Oncol Biol Phys 58, 984-90.
- Sridhar, S.S. et al. (2003) Lancet Oncol 4, 397-406.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Applications Key
IF-P: Immunofluorescence (Paraffin) IF-IC: Immunofluorescence (Immunocytochemistry)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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