WB, IP
H M R
Endogenous
130, 120
Rabbit
#Q8IUD2
23085
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat
Species predicted to react based on 100% sequence homology
The antigen sequence used to produce this antibody shares
100% sequence homology with the species listed here, but
reactivity has not been tested or confirmed to work by CST.
Use of this product with these species is not covered under
our
Product Performance Guarantee.
Monkey
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of ERC1 protein. Antibodies are purified by peptide affinity chromatography.
Background
ERC1, an acronym named for previous protein names ELKS (1), RAB6IP2 (2) and CAST (3), is a RIM-binding protein that plays a role in neurotransmitter release and general membrane trafficking in other cell types (2-5). Interaction with the GTP-binding protein Rab6 suggests that it contributes to membrane traffic at the Golgi (2). In addition to its association with membrane trafficking, ERC1 has also been found as an essential part of the IκB kinase (IKK) complex required for the activation of NF-κB, perhaps by recruiting IκBα to the IKK complex (6). Alternative splicing of ERC1 generates 2 proteins with a divergent carboxy terminus, a long and a short form termed ERC1α and ERC1β, respectively. ERC1α is widely expressed, whereas ERC1β and a related family member ERC2 are expressed in the brain (4). Papillary thyroid carcinomas have been identified with the translocation t(10;12)(p11;p13) resulting in a fusion between ERC1 and the receptor tyrosine kinase Ret (1).
- Nakata, T. et al. (1999) Genes Chromosomes Cancer 25, 97-103.
- Monier, S. et al. (2002) Traffic 3, 289-97.
- Ohtsuka, T. et al. (2002) J Cell Biol 158, 577-90.
- Wang, Y. et al. (2002) Proc Natl Acad Sci USA 99, 14464-9.
- Ohara-Imaizumi, M. et al. (2005) Mol Biol Cell 16, 3289-300.
- Ducut Sigala, J.L. et al. (2004) Science 304, 1963-7.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
Limited Uses
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