WB, FC-FP
H M R Mk
Endogenous
68-80
Rabbit
#Q6UN15
81608
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Flow Cytometry (Fixed/Permeabilized) | 1:25 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Phe164 of human FIP1L1. Antibodies are purified by protein A and peptide affinity chromatography.
Background
FIP1L1 is the mammalian homolog of yeast Fip1p (factor interacting with poly(A) polymerase (PAP)). It is a subunit of cleavage and polyadenylation specificity factor (CPSF) that binds to PAP and to U-rich sequences on the pre-mRNA, stimulating polyadenylation of pre-mRNA (1). FIP1L1 has several splicing variants (1). FIP1L1 is fused to platelet-derived growth factor receptor α gene (PDGFRα) by an interstitial deletion on chromosome 4q12 in some patients with idiopathic hypereosinophilic syndrome (HES) and chronic eosinophilic leukemia (CEL). Τhe resulting FIP1L1-PDGFRα fusion protein has constitutively elevated tyrosine kinase activity (2).
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting FC-FP: Flow Cytometry (Fixed/Permeabilized)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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