WB, IP, IF-F, IF-IC
H M R
Endogenous
17
Rabbit IgG
#Q9Y3D6
51024
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| Immunofluorescence (Frozen) | 1:200 - 1:800 |
| Immunofluorescence (Immunocytochemistry) | 1:200 - 1:800 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human FIS1 protein.
Background
Changes in mitochondrial dynamics regulated by environmental cues affect mitochondrial size and shape and have been shown to dramatically impact mitochondrial metabolism, apoptosis, and autophagy (1). Mitochondrial fusion (elongation) and fission (fragmentation) are dynamically controlled by several interacting proteins. Mitochondrial fission is controlled by the GTPase DRP1 which is recruited to the mitochondria by FIS1 and MFF. FIS1 is localized to the mitochondrial outer membrane and overexpression induces fission and leads to apoptosis (2-4). Decreased expression of FIS1 leads to mitochondrial elongation and triggers cellular senescence (5). Elevated expression of FIS1 can also promote autophagy (6). Studies also found that FIS1 interacts with the autophagy initiation protein STX17 and that depletion of FIS1 leads to an increase in mitochondrial localized STX17 and mitophagy (7). Expression of FIS1 has been associated with neurodegeneration and disease, including cancer (8).
- Suen, D.F. et al. (2008) Genes Dev 22, 1577-90.
- James, D.I. et al. (2003) J Biol Chem 278, 36373-9.
- Lee, Y.J. et al. (2004) Mol Biol Cell 15, 5001-11.
- Stojanovski, D. et al. (2004) J Cell Sci 117, 1201-10.
- Lee, S. et al. (2007) J Biol Chem 282, 22977-83.
- Gomes, L.C. and Scorrano, L. Biochim Biophys Acta 1777, 860-6.
- Xian, H. et al. (2019) Nat Commun 10, 2059.
- Serasinghe, M.N. and Chipuk, J.E. (2017) Handb Exp Pharmacol 240, 159-188.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation IF-F: Immunofluorescence (Frozen) IF-IC: Immunofluorescence (Immunocytochemistry)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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