| Product Includes | Product # | Quantity | Mol. Wt | Isotype/Source |
|---|---|---|---|---|
| mGluR1 (D5H10) Rabbit mAb | 12551 | 20 µl | 145, >300 kDa | Rabbit IgG |
| mGluR5 (D6E7B) Rabbit mAb | 55920 | 20 µl | 150, 300 kDa | Rabbit IgG |
| FMRP (D14F4) Rabbit mAb | 7104 | 20 µl | 80 kDa | Rabbit IgG |
| FXR1 (D10A2) XP® Rabbit mAb | 12295 | 20 µl | 78-80, 82-84 kDa | Rabbit IgG |
| FXR2 (D85D6) Rabbit mAb | 7098 | 20 µl | 95 kDa | Rabbit IgG |
| CYFIP1 Antibody | 44353 | 20 µl | 145 kDa | Rabbit |
| Phospho-eEF2 (Thr56) Antibody | 2331 | 20 µl | 95 kDa | Rabbit |
| eEF2 Antibody | 2332 | 20 µl | 95 kDa | Rabbit |
| Anti-rabbit IgG, HRP-linked Antibody | 7074 | 100 µl | Goat |
Please visit cellsignal.com for individual component applications, species cross-reactivity, dilutions, protocols, and additional product information.
Description
The Fragile X/FMRP Signaling Pathway Antibody Sampler Kit provides an economical means of detecting signaling components of the Fragile X/FMRP signaling pathway. The kit includes enough antibodies to perform two western blot experiments with each primary antibody.
Storage
Background
Fragile X syndrome, a frequent cause of inherited mental retardation, often results from expansion of the CGG trinucleotide repeat in the gene that encodes the fragile X mental retardation protein (FMRP, [1]). FMRP (also known as FMR1) and its two autosomal homologs (FXR1 and FXR2) all bind RNA and play a role in the pathogenesis of fragile X syndrome (1-3). Each of these related proteins can associate with one another as well as form homodimers and complexes with other RNA-binding proteins like cytoplasmic FMRP interacting protein 1 (CYFIP1, [3,4]). FMRP, FXR1, FXR2, and CYFIP1 have been implicated in the translational regulation of mRNAs (5,6). Importantly, this complex of proteins may be dynamically regulated to drive protein synthesis-dependent forms of synaptic plasticity in response to specific activity. That is, activation of metabotropic glutamate receptors, including mGluR1 and mGlur5, can regulate FMRP-dependent forms of translation via post-translational modification of eukaryotic elongation factor 2 (eEF2) to locally control dynamic translation of important synaptic proteins, which, subsequently, alter synaptic function (7-9).
- Verkerk, A.J. et al. (1991) Cell 65, 905-14.
- Siomi, M.C. et al. (1995) EMBO J 14, 2401-8.
- Zhang, Y. et al. (1995) EMBO J 14, 5358-66.
- Abekhoukh, S. et al. (2017) Dis Model Mech 10, 463-74.
- Linder, B. et al. (2008) Hum Mol Genet 17, 3236-46.
- De Rubeis, S. et al. (2013) Neuron 79, 1169-82.
- Park, S. et al. (2008) Neuron 59, 70-83.
- Barnes, S.A. et al. (2015) J Neurosci 35, 15073-81.
- Paul, A. et al. (2019) Front Mol Neurosci 12, 97.
Background References
Trademarks and Patents
Limited Uses
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