WB, IP, ChIP
H M
Endogenous
55
Mouse IgG2a
#Q96RI1
9971
Product Information
Product Usage Information
For optimal ChIP results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 × 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:200 |
| Chromatin IP | 1:50 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human FXR/NR1H4 protein.
Background
The farnesoid X receptor (FXR/NR1H4) is a member of the nuclear hormone receptor superfamily and is a master regulator of bile acid synthesis. FXR/NR1H4 heterodimerizes with RXR-alpha upon activation by bile acids, which begins a regulatory cascade involving SHP and LRH-1 to control lipid homeostasis (1-5). FXR/NR1H4 has also been shown to be a critical regulator of glucose homeostasis (6,7). In addition to directly regulating genes, FXR/NR1H4 also plays a post transcriptional role in bile acid metabolism by transcribing the RNA-binding protein ZFP36L1, which in turn downregulates the key enzyme Cyp7a1 (8). Mutations in human FXR/NR1H4 have been shown to cause cholestasis and liver disease in neonatal patients (9). FXR/NR1H4 can also control Lgr5+ intestinal stem cell proliferation and its upregulation has been shown to inhibit colorectal cancer progression (10-12). Agonists against FXR/NR1H4 are being evaluated for various liver diseases and diabetes (13-16).
- Parks, D.J. et al. (1999) Science 284, 1365-8.
- Wang, H. et al. (1999) Mol Cell 3, 543-53.
- Laffitte, B.A. et al. (2000) J Biol Chem 275, 10638-47.
- Edwards, P.A. et al. (2002) J Lipid Res 43, 2-12.
- Goodwin, B. et al. (2000) Mol Cell 6, 517-26.
- Ma, K. et al. (2006) J Clin Invest 116, 1102-9.
- Zhang, Y. et al. (2006) Proc Natl Acad Sci U S A 103, 1006-11.
- Tarling, E.J. et al. (2017) J Clin Invest 127, 3741-54.
- Gomez-Ospina, N. et al. (2016) Nat Commun 7, 10713.
- Modica, S. et al. (2008) Cancer Res 68, 9589-94.
- Fu, T. et al. (2019) Cell 176, 1098-1112.e18.
- Qiao, P. et al. (2018) Oncol Rep 40, 2067-78.
- Pellicciari, R. et al. (2002) J Med Chem 45, 3569-72.
- Hirschfield, G.M. et al. (2015) Gastroenterology 148, 751-61.e8.
- Neuschwander-Tetri, B.A. et al. (2015) Lancet 385, 956-65.
- Genin, M.J. et al. (2015) J Med Chem 58, 9768-72.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation ChIP: Chromatin IP
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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