WB, IP
H
Endogenous
30,32
Rabbit
#Q96F15
55340
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human GIMAP5 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
GTPase immune-associated proteins (GIMAP), also known as immune-associated nucleotide-binding (IAN) proteins, are evolutionarily conserved GTP-binding proteins involved in lymphocyte development, inflammation, and autoimmune diseases (reviewed in 1,2). Human GTPase IMAP family member 5 (GIMAP5, hIan5) is the homolog of the rat Ian4 protein that is mutated in severe cases of T-cell lymphopenia and insulin-dependent diabetes in Biobreeding diabetes-prone (BB-DP) rats (3,4). GIMAP5 protein is preferentially expressed in CD4- and CD8-positive T-cells as well as B-cell lymphomas (4). Research studies using GIMAP5-deficient mice show that GIMAP5 protein is critical for survival of peripheral T-cells, hematopoietic stem cells, and progenitor cells (5-7). Additional studies indicate that GIMAP5 deficiency leads to a loss of immunological tolerance (8). Polymorphisms in the human GIMAP5 gene are associated with systemic lupus erythematosus and type I diabetes (9-11). Potential mechanisms for GIMAP5 control of cell survival include regulation of Bcl-2 family proteins, mitochondrial integrity, lysosomal function, and calcium regulation (5,7, 11-14).
- Nitta, T. and Takahama, Y. (2007) Trends Immunol 28, 58-65.
- Ciucci, T. and Bosselut, R. (2014) Eur J Immunol 44, 348-51.
- Hornum, L. et al. (2002) Diabetes 51, 1972-9.
- Zenz, T. et al. (2004) Genes Immun 5, 109-16.
- Pandarpurkar, M. et al. (2003) Proc Natl Acad Sci U S A 100, 10382-7.
- Schulteis, R.D. et al. (2008) Blood 112, 4905-14.
- Chen, Y. et al. (2011) J Exp Med 208, 923-35.
- Aksoylar, H.I. et al. (2012) J Immunol 188, 146-54.
- Hellquist, A. et al. (2007) J Med Genet 44, 314-21.
- Shin, J.H. et al. (2007) Genes Immun 8, 503-12.
- Lim, M.K. et al. (2009) Lupus 18, 1045-52.
- Keita, M. et al. (2007) Biochem Biophys Res Commun 361, 481-6.
- Wong, V.W. et al. (2010) Self Nonself 1, 259-268.
- Chen, X.L. et al. (2013) Biochem J 449, 353-64.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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