WB, IP
H M R
Endogenous
46
Rabbit IgG
#P48051
3763
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu22 of human GIRK2 protein.
Background
GIRK2 is a member of G protein-coupled inwardly rectifying potassium channel family proteins (GIRKs). GIRK family proteins allow potassium to flow into the cell and therefore control cellular excitability in the central nervous system, heart, and pancreas (1-4). Activation of most GIRK channels requires heterologous subunit assembly and the presence of ATP (5-7). GIRK2 is abundantly expressed in the brain, where it is involved in pain perception. It is also required for peripheral opioid-mediated analgesia (8). Additionally GIRK2 localizes to pancreatic β cells and regulates insulin secretion (9,10). Mutations in the KCNJ6 gene encoding GIRK2 are associated with Keppen-Lubinsky Syndrome, a rare disease characterized by severe developmental delay, facial dysmorphism, and intellectual disability (11).
- Lesage, F. et al. (1995) J Biol Chem 270, 28660-7.
- Kubo, Y. et al. (1993) Nature 364, 802-6.
- Krapivinsky, G. et al. (1995) Nature 374, 135-41.
- Isomoto, S. et al. (1997) Jpn J Physiol 47, 11-39.
- Duprat, F. et al. (1995) Biochem Biophys Res Commun 212, 657-63.
- Corey, S. and Clapham, D.E. (1998) J Biol Chem 273, 27499-504.
- Han, J. et al. (2003) J Physiol 550, 693-706.
- Nockemann, D. et al. (2013) EMBO Mol Med 5, 1263-77.
- Ferrer, J. et al. (1995) J Biol Chem 270, 26086-91.
- Sakura, H. et al. (1995) FEBS Lett 367, 193-7.
- Masotti, A. et al. (2015) Am J Hum Genet 96, 295-300.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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