WB, IP
H M R Mk
Endogenous
49
Mouse IgG2a
#P50502
6767
Product Information
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Immunoprecipitation | 1:50 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to rat Hip protein.
Background
Hip (HSP70-interacting protein), also known as ST13 (suppression of tumorigenicity protein 13), is one of several co-chaperones that regulate activities of the HSP70 chaperone family (1,2). The homo-oligomeric protein Hip cooperates with HSP70 in protein folding by stabilizing the ADP-bound state of HSP70. Hip directly binds to the ATPase domain of HSP70 when it is converted to the ADP-bound state by proteins of the HSP40 family (3). By collaborating with other positive co-factors such as HSP40 and Hop, or competing with negative co-factors such as Bag1, Hip may facilitate the chaperone function of HSP70 in protein folding and repair, and in controlling the activity of regulatory proteins such as steroid receptors and various regulators of proliferation or apoptosis (4-8).
- Prapapanich, V. et al. (1996) Mol. Endocrino.l 10, 420-431.
- Gebauer, M. et al. (1997) FEBS Lett. 417, 109-113.
- Höhfeld, J. et al. (1995) Cell 83, 589-.98.
- Frydman, J. and Höhfeld, J. (1997) Trends Biochem. Sci. 22, 87-92.
- Nollen, E.A. et al. (2001) J. Biol. Chem. 276, 4677-4682.
- Fan, G.H. et al. (2002) J. Biol. Chem. 277, 6590-6597.
- Nelson, G.M. et al. (2004) Mol. Endocrinol. 18, 1620-1630.
- Shi, Z.Z. et al. (2007) J. Zhejiang Univ. Sci. B. 8, 170-176.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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