Revision 1

#9928

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Orders:

877-616-CELL (2355)

[email protected]

Support:

877-678-TECH (8324)

3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

Product Includes Product # Quantity Mol. Wt Isotype/Source
HDAC1 (10E2) Mouse Monoclonal Antibody535620 µl62 kDaMouse IgG1
HDAC2 (3F3) Mouse Monoclonal Antibody511320 µl60 kDaMouse IgG1 kappa
HDAC3 (7G6C5) Mouse Monoclonal Antibody394920 µl49 kDaMouse IgG2a
HDAC4 (D15C3) Rabbit Monoclonal Antibody762820 µl140 kDaRabbit IgG
Phospho-HDAC4 (Ser246)/HDAC5 (Ser259)/HDAC7 (Ser155) (D27B5) Rabbit Monoclonal Antibody344320 µl140, 124 kDaRabbit IgG
HDAC6 (D2E5) Rabbit Monoclonal Antibody755820 µl160 kDaRabbit IgG
Anti-rabbit IgG, HRP-linked Antibody7074100 µlGoat
Anti-mouse IgG, HRP-linked Antibody7076100 µlHorse

Please visit cellsignal.com for individual component applications, species cross-reactivity, dilutions, protocols, and additional product information.

Description

The Histone Deacetylase (HDAC) Antibody Sampler Kit provides a fast and economical means to evaluate the endogenous levels of HDACs. The kit contains enough primary and secondary antibodies to perform two Western blot experiments.

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Background

Acetylation of the histone tail causes chromatin to adopt an "open" conformation, allowing increased accessibility of transcription factors to DNA. The identification of histone acetyltransferases (HATs) and their large multiprotein complexes has yielded important insights into how these enzymes regulate transcription (1,2). HAT complexes interact with sequence-specific activator proteins to target specific genes. In addition to histones, HATs can acetylate nonhistone proteins, suggesting multiple roles for these enzymes (3). In contrast, histone deacetylation promotes a "closed" chromatin conformation and typically leads to repression of gene activity (4). Mammalian histone deacetylases can be divided into three classes on the basis of their similarity to various yeast deacetylases (5). Class I proteins (HDACs 1, 2, 3, and 8) are related to the yeast Rpd3-like proteins, those in class II (HDACs 4, 5, 6, 7, 9, and 10) are related to yeast Hda1-like proteins, and class III proteins are related to the yeast protein Sir2. Inhibitors of HDAC activity are now being explored as potential therapeutic cancer agents (6,7).

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Western blot analysis of NIH/3T3 cells using Phospho-HDAC4 (Ser246)/HDAC5 (Ser259)/HDAC7 (Ser155) (D27B5) Rabbit mAb #3443, HDAC4 (D8T3Q) Rabbit mAb #15164, HDAC5 (D1J7V) Rabbit mAb #20458, and HDAC7 (D4E1L) Rabbit mAb #33418. To show the phospho-specificity of the antibody, the blot was mock treated (-) or treated (+) with calf intestinal phosphatase (CIP) and lambda phosphatase. As expected, signal from the Phospho-HDAC4 (Ser246)/HDAC5 (Ser259)/HDAC7 (Ser155) (D27B5) Rabbit mAb is lost after treatment of the blot with phosphatase.
undefined Image 1: Histone Deacetylase (HDAC) Antibody Sampler Kit
Western blot analysis of extracts from various cell lines using HDAC3 (7G6C5) Mouse mAb.
undefined Image 2: Histone Deacetylase (HDAC) Antibody Sampler Kit
Western blot analysis of extracts from various cell lines using HDAC3 (7G6C5) Mouse mAb.
undefined Image 3: Histone Deacetylase (HDAC) Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using HDAC2 (3F3) Mouse mAb.
undefined Image 4: Histone Deacetylase (HDAC) Antibody Sampler Kit
Western blot analysis of extracts from HeLa, NIH/3T3, and H-4-II-E cells using HDAC1 (10E2) Mouse mAb.
undefined Image 5: Histone Deacetylase (HDAC) Antibody Sampler Kit
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.
undefined Image 6: Histone Deacetylase (HDAC) Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO* is added and emits light during enzyme catalyzed decomposition.
undefined Image 7: Histone Deacetylase (HDAC) Antibody Sampler Kit
Western blot analysis of extracts from various cell lines using HDAC6 (D2E5) Rabbit mAb.
undefined Image 8: Histone Deacetylase (HDAC) Antibody Sampler Kit
Western blot analysis of extracts from various cell lines using HDAC4 (D15C3) Rabbit mAb.
undefined Image 9: Histone Deacetylase (HDAC) Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Confocal immunofluorescent analysis of HeLa cells using HDAC3 (7G6C5) Mouse mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red).
undefined Image 10: Histone Deacetylase (HDAC) Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma using HDAC2 (3F3) Mouse mAb.
undefined Image 11: Histone Deacetylase (HDAC) Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using HDAC6 (D2E5) Rabbit mAb.
undefined Image 12: Histone Deacetylase (HDAC) Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Immunohistochemical analysis of paraffin-embedded human B-cell non-Hodgkin lymphoma using HDAC2 (3F3) Mouse mAb.
undefined Image 13: Histone Deacetylase (HDAC) Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using HDAC6 (D2E5) Rabbit mAb.
undefined Image 14: Histone Deacetylase (HDAC) Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded normal human esophagus using HDAC2 (3F3) Mouse mAb.
undefined Image 15: Histone Deacetylase (HDAC) Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Confocal immunofluorescent analysis of A549 cells, untreated (left) or treated with MG132 (5 μM, 24 hr; right), using HDAC6 (D2E5) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5®#4084 (fluorescent DNA dye).
undefined Image 16: Histone Deacetylase (HDAC) Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded normal human uterus using HDAC2 (3F3) Mouse mAb.
undefined Image 17: Histone Deacetylase (HDAC) Antibody Sampler Kit
Flow cytometric analysis of K562 cells using HDAC6 (D2E5) Rabbit mAb (solid line) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed line). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.
undefined Image 18: Histone Deacetylase (HDAC) Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Immunohistochemical analysis of paraffin-embedded A20 syngeneic tumor using HDAC2 (3F3) Mouse mAb.
undefined Image 19: Histone Deacetylase (HDAC) Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded mouse cerebellum using HDAC2 (3F3) Mouse mAb.
undefined Image 20: Histone Deacetylase (HDAC) Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded non-small cell lung carcinoma using HDAC2 (3F3) Mouse mAb (left) or an HDAC2 antibody (right). These two antibodies detect unique, non-overlapping epitopes on HDAC2. The similar staining patterns obtained with both antibodies help to confirm the specificity of the staining.
undefined Image 21: Histone Deacetylase (HDAC) Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Immunohistochemical analysis of paraffin-embedded human non-small cell lung carcinoma using HDAC2 (3F3) Mouse mAb (left) compared to concentration-matched Mouse (G3A1) mAb IgG1 Isotype Control #5415 (right).
undefined Image 22: Histone Deacetylase (HDAC) Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded HCT 116 cell pellets, control (left, positive) or CRISPR/Cas9 HDAC2 knockout (KO) (right, negative), using HDAC2 (3F3) Mouse mAb.
undefined Image 23: Histone Deacetylase (HDAC) Antibody Sampler Kit
Western blot analysis of extracts from various cell lines using HDAC2 (3F3) Mouse mAb.
undefined Image 24: Histone Deacetylase (HDAC) Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Confocal immunofluorescent analysis of HeLa cells using HDAC2 (3F3) Mouse mAb (green). Actin filaments were labeled using DY-554 Phalloidin (red).
undefined Image 25: Histone Deacetylase (HDAC) Antibody Sampler Kit
Immunoprecipitation of HDAC2 protein from HeLa cell extracts. Lane 1 is 10% input, lane 2 is Mouse (G3A1) mAb IgG1 Isotype Control #5415, and lane 3 is HDAC2 (3F3) Mouse mAb. Western blot analysis was performed using HDAC2 (D6S5P) Rabbit mAb #57156.
undefined Image 26: Histone Deacetylase (HDAC) Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.