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9928
Histone Deacetylase (HDAC) Antibody Sampler Kit

Histone Deacetylase (HDAC) Antibody Sampler Kit #9928

Western Blotting Image 1

Western blot analysis of extracts from HeLa, NIH/3T3, and H-4-II-E cells using HDAC1 (10E2) Mouse mAb.

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Western Blotting Image 2

Western blot analysis of extracts from various cell lines using HDAC2 (3F3) Mouse mAb.

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Western Blotting Image 3

Western blot analysis of extracts from various cell lines using HDAC3 (7G6C5) Mouse mAb.

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Western Blotting Image 4

Western blot analysis of extracts from various cell lines using HDAC4 (D15C3) Rabbit mAb.

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Western Blotting Image 5

Western blot analysis of NIH/3T3 cells using Phospho-HDAC4 (Ser246)/HDAC5 (Ser259)/HDAC7 (Ser155) (D27B5)

Rabbit mAb #3443, HDAC4 (D8T3Q) Rabbit mAb #15164, HDAC5 (D1J7V) Rabbit mAb #20458, and HDAC7 (D4E1L) Rabbit mAb #33418. To show the phospho-specificity of the antibody, the blot was mock treated (-) or treated (+) with calf intestinal phosphatase (CIP) and lambda phosphatase. As expected, signal from the Phospho-HDAC4 (Ser246)/HDAC5 (Ser259)/HDAC7 (Ser155) (D27B5) Rabbit mAb is lost after treatment of the blot with phosphatase.

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Western Blotting Image 6

Western blot analysis of extracts from various cell lines using HDAC6 (D2E5) Rabbit mAb.

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Western Blotting Image 7

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

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IF-IC Image 8

Confocal immunofluorescent analysis of HeLa cells using HDAC2 (3F3) Mouse mAb (green). Actin filaments were labeled using DY-554 Phalloidin (red).

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IF-IC Image 9

Confocal immunofluorescent analysis of HeLa cells using HDAC3 (7G6C5) Mouse mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red).

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IHC-P (paraffin) Image 10

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using HDAC6 (D2E5) Rabbit mAb.

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IHC-P (paraffin) Image 11

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using HDAC6 (D2E5) Rabbit mAb.

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Flow Cytometry Image 12

Flow cytometric analysis of K562 cells using HDAC6 (D2E5) Rabbit mAb (solid line) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed line). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.

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IF-IC Image 13

Confocal immunofluorescent analysis of A549 cells, untreated (left) or treated with MG132 (5 μM, 24 hr; right), using HDAC6 (D2E5) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5®#4084 (fluorescent DNA dye).

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Product Includes Quantity Applications Reactivity MW(kDa) Isotype
HDAC1 (10E2) Mouse mAb 5356 20 µl
  • WB
  • IP
H M R Mk 62 Mouse IgG1
HDAC2 (3F3) Mouse mAb 5113 20 µl
  • WB
  • IP
  • IF
H M R Mk 60 Mouse IgG1
HDAC3 (7G6C5) Mouse mAb 3949 20 µl
  • WB
  • IP
  • IF
H M R Mk 49 Mouse IgG2a
HDAC4 (D15C3) Rabbit mAb 7628 20 µl
  • WB
  • IP
H M R Mk 140 Rabbit IgG
Phospho-HDAC4 (Ser246)/HDAC5 (Ser259)/HDAC7 (Ser155) (D27B5) Rabbit mAb 3443 20 µl
  • WB
H M 140, 124 Rabbit IgG
HDAC6 (D2E5) Rabbit mAb 7558 20 µl
  • WB
  • IP
  • IHC
  • IF
  • F
H Mk 160 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
  • WB
Goat 
Anti-mouse IgG, HRP-linked Antibody 7076 100 µl
  • WB
Horse 

The Histone Deacetylase (HDAC) Antibody Sampler Kit provides a fast and economical means to evaluate the endogenous levels of HDACs. The kit contains enough primary and secondary antibodies to perform two Western blot experiments.

The Histone Deacetylase (HDAC) Antibody Sampler Kit provides a fast and economical means to evaluate the endogenous levels of HDACs. The kit contains enough primary and secondary antibodies to perform four mini-blot experiments.

Each antibody in the Histone Deacetylase (HDAC) Antibody Sampler Kit detects endogenous levels of its target.

Monoclonal antibodies are produced by immunizing animals with a recombinant protein specific to the carboxy terminus of human HDAC6, the amino terminus of human HDAC4 protein, synthetic peptides to the carboxy-terminal residues of human HDAC1 and HDAC2 proteins, or synthetic phosphopeptide corresponding to Ser155 of human HDAC7 protein. HDAC3 (7G6C5) monoclonal antibody is produced by immunizing animals with recombinant human HDAC3 protein.

Acetylation of the histone tail causes chromatin to adopt an "open" conformation, allowing increased accessibility of transcription factors to DNA. The identification of histone acetyltransferases (HATs) and their large multiprotein complexes has yielded important insights into how these enzymes regulate transcription (1,2). HAT complexes interact with sequence-specific activator proteins to target specific genes. In addition to histones, HATs can acetylate nonhistone proteins, suggesting multiple roles for these enzymes (3). In contrast, histone deacetylation promotes a "closed" chromatin conformation and typically leads to repression of gene activity (4). Mammalian histone deacetylases can be divided into three classes on the basis of their similarity to various yeast deacetylases (5). Class I proteins (HDACs 1, 2, 3, and 8) are related to the yeast Rpd3-like proteins, those in class II (HDACs 4, 5, 6, 7, 9, and 10) are related to yeast Hda1-like proteins, and class III proteins are related to the yeast protein Sir2. Inhibitors of HDAC activity are now being explored as potential therapeutic cancer agents (6,7).

  1. Marmorstein, R. (2001) Cell Mol Life Sci 58, 693-703.
  2. Gregory, P.D. et al. (2001) Exp Cell Res 265, 195-202.
  3. Liu, Y. et al. (2000) Mol Cell Biol 20, 5540-53.
  4. Cress, W.D. and Seto, E. (2000) J Cell Physiol 184, 1-16.
  5. Gray, S.G. and Ekström, T.J. (2001) Exp Cell Res 262, 75-83.
  6. Thiagalingam, S. et al. (2003) Ann. N.Y. Acad. Sci. 983, 84-100.
  7. Vigushin, D.M. and Coombes, R.C. (2004) Curr Cancer Drug Targets 4, 205-18.
Entrez-Gene Id
3065 , 3066 , 8841 , 9759 , 10014 , 10013 , 51564
Swiss-Prot Acc.
Q13547 , Q92769 , O15379 , P56524 , Q9UQL6 , Q9UBN7 , Q8WUI4
For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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