WB
H Mk
Endogenous
40
Rabbit IgG
#P07910
3183
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ile217 of human hnRNP C1/C2 protein.
Background
Heterogeneous nuclear ribonucleoprotein C1/C2 (hnRNP C1/C2) has multiple biological functions including transcriptional regulation, DNA repair, and RNA processing. hnRNP C1/C2 acts as a ‘molecular ruler’ in the mRNA processing pathway, committing nascent transcripts from the chromatin template to the mRNA export pathway once the nascent transcript becomes longer than 200-300 nucleotides (1). hnRNP C1/C2 associates with SWI/SNF and NurD family members to form the locus control region (LCR)-associated remodeling complex (LARC), which binds to β-globin gene promoter to prevent transcriptional silencing. Studies indicate that without hnRNP C1/C2, LARC does not associate with its target DNA sequence (2,3). hnRNP C1/C2 and other hnRNP family members interact with DNA damage response (DDR) proteins (4). hnRNP proteins regulate double stranded break (DSB) repair by promoting either homologous recombination (HR) or non-homologous end joining (NHEJ) (4). hnRNP C1/C2 downregulates the expression of miR-21, which leads to the increased expression of programmed cell death 4 (PDCD4) protein in glioblastoma multiforme (GBM) (5). Research studies have shown that silencing of hnRNP C1/C2 renders GBM cells more susceptible to apoptosis (5).
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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