WB
H
Endogenous
78
Rabbit
#P52272
4670
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro43 of human hnRNP M protein. Antibodies are purified by peptide affinity chromatography.
Background
Heterogenous nuclear ribonucleoprotein M (hnRNP M) belongs to a family of RNA-binding multi-protein complexes (hnRNP proteins) that facilitate pre-mRNA processing (1,2). hnRNP M binds to GU rich regions of RNA and exists as 4 isoforms named M1-M4 (2). Binding to pre-mRNA in early stages of spliceosome assembly, hnRNP M contributes to splicing events by inducing exon skipping as well as promoting exon inclusion (3,4). hnRNP M is expressed on the surface of Kupffer cells, and acts as a receptor for carcinoembryonic antigen (CEA), a biomarker for colorectal cancer metastasis to the liver (5-7). hnRNP M has also shown to be a key regulator of the innate immune response in macrophages, where it represses key inflammatory and antimicrobial genes (8,9).
- Dreyfuss, G. et al. (1993) Annu Rev Biochem 62, 289-321.
- Datar, K.V. et al. (1993) Nucleic Acids Res 21, 439-46.
- Kafasla, P. et al. (2002) Biochem J 363, 793-9.
- Hovhannisyan, R.H. and Carstens, R.P. (2007) J Biol Chem 282, 36265-74.
- Bajenova, O. et al. (2003) Exp Cell Res 291, 228-41.
- Laguinge, L. et al. Anticancer Res 25, 23-31.
- Thomas, P. et al. (2011) Clin Exp Metastasis 28, 923-32.
- Cao, P. et al. (2019) PLoS Pathog 15, e1007983.
- West, K.O. et al. (2019) Cell Rep 29, 1594-1609.e5.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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