hnRNP M Antibody #28699
- WB
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 78 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Protocol
Specificity / Sensitivity
hnRNP M Antibody recognizes endogenous levels of total hnRNP M protein.
Species Reactivity:
Human
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro43 of human hnRNP M protein. Antibodies are purified by peptide affinity chromatography.
Background
Heterogenous nuclear ribonucleoprotein M (hnRNP M) belongs to a family of RNA-binding multi-protein complexes (hnRNP proteins) that facilitate pre-mRNA processing (1,2). hnRNP M binds to GU rich regions of RNA and exists as 4 isoforms named M1-M4 (2). Binding to pre-mRNA in early stages of spliceosome assembly, hnRNP M contributes to splicing events by inducing exon skipping as well as promoting exon inclusion (3,4). hnRNP M is expressed on the surface of Kupffer cells, and acts as a receptor for carcinoembryonic antigen (CEA), a biomarker for colorectal cancer metastasis to the liver (5-7). hnRNP M has also shown to be a key regulator of the innate immune response in macrophages, where it represses key inflammatory and antimicrobial genes (8,9).
- Dreyfuss, G. et al. (1993) Annu Rev Biochem 62, 289-321.
- Datar, K.V. et al. (1993) Nucleic Acids Res 21, 439-46.
- Kafasla, P. et al. (2002) Biochem J 363, 793-9.
- Hovhannisyan, R.H. and Carstens, R.P. (2007) J Biol Chem 282, 36265-74.
- Bajenova, O. et al. (2003) Exp Cell Res 291, 228-41.
- Laguinge, L. et al. Anticancer Res 25, 23-31.
- Thomas, P. et al. (2011) Clin Exp Metastasis 28, 923-32.
- Cao, P. et al. (2019) PLoS Pathog 15, e1007983.
- West, K.O. et al. (2019) Cell Rep 29, 1594-1609.e5.
Limited Uses
Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.
Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.