WB, IP, ChIP
H Mk
Endogenous
42
Rabbit IgG
#P28356
3235
Product Information
Product Usage Information
For optimal ChIP results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 × 10^6 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| Chromatin IP | 1:50 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly159 of human HOXD9 protein.
Background
Homeobox protein Hox-D9 (HOXD9) is a sequence-specific transcription factor that is part of a developmental regulatory program that provides cells with specific positional identities on the anterior-posterior axis. HOXD9 is developmentally expressed in structures of either mesodermal or neuro-ectodermal origin, such as developing limbs, gonads, and the central nervous system (1-6). HOXD9 plays a critical role in regulation of limb development, neuronal development, and development of mammary glands and gonads in many organisms (1-6). The HOXD9 gene promoter is found to be hypermethylated and silenced in multiple types of cancer, including breast cancer, melanoma brain metastases, and cholangiocarcinomas (7-9). In addition, HOXD9 expression is increased in other types of cancer, including human glioblastomas and astrocytomas, where expression appears to drive growth of the tumors (10).
- Raines, A.M. et al. (2013) Development 140, 2942-52.
- Wang, Z. et al. (2010) BMC Genomics 11, 619.
- Freitas, R. et al. (2007) PLoS One 2, e754.
- Tanaka, M. et al. (2005) Dev Biol 281, 227-39.
- de la Cruz, C.C. et al. (1999) Dev Biol 216, 595-610.
- Chen, F. and Capecchi, M.R. (1999) Proc Natl Acad Sci U S A 96, 541-6.
- Makiyama, K. et al. (2005) Oncol Rep 13, 673-9.
- Marzese, D.M. et al. (2014) Hum Mol Genet 23, 226-38.
- Sriraksa, R. et al. (2013) Cancer Prev Res (Phila) 6, 1348-55.
- Tabuse, M. et al. (2011) Mol Cancer 10, 60.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation ChIP: Chromatin IP
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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