WB
H M R Mk
Endogenous
40
Rabbit
#O00221
4794
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the middle of human IκBε. Antibodies are purified by protein A and peptide affinity chromatography.
Product Description
| MW (kDa) | 40 |
| Activity | ****** The range listed in PDP for this antigen is 59-70. This does not match the most commonly identified human entries in a BLAST search (for most BLAST returns the antigen sequence matched Human IkB epsilon 199-209). The 59-70 notation does not correlate with other standard database entries for human IkB epsilon - including the uniprot enrty (http://www.uniprot.org/uniprot/O00221). For this antigen the correct notation is residues 199-209, residues 59-70 (as currently listed) is incorrect. Note Q198 has been substituted with a cysteine in the immunizing antigen (Q198C, thus 198-208?). Also note the webpage and datasheet state: "Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the middle of human IκBε". This information is correct and consistent with the antigen at residues 199-209. The peptide notebook will remain incorrect. |
Background
The NF-κB/Rel transcription factors are present in the cytosol in an inactive state complexed with the inhibitory IκB proteins (1-3). Activation occurs via phosphorylation of IκBα at Ser32 and Ser36 followed by proteasome-mediated degradation that results in the release and nuclear translocation of active NF-κB (3-7). IκBα phosphorylation and resulting Rel-dependent transcription are activated by a highly diverse group of extracellular signals including inflammatory cytokines, growth factors, and chemokines. Kinases that phosphorylate IκB at these activating sites have been identified (8).
The regulation of IκBβ and IκBε is similar to that of IκB-α. However, the phosphorylation and ubiquitin-mediated degradation of these proteins occurs with much slower kinetics (9). IKK phosphorylation of IκB-β occurs at Ser19 and Ser23, while IκBε can be phosphorylated at Ser18 and Ser22 (10).
- Baeuerle, P.A. and Baltimore, D. (1988) Science 242, 540-6.
- Beg, A.A. and Baldwin, A.S. (1993) Genes Dev 7, 2064-70.
- Finco, T.S. et al. (1994) Proc Natl Acad Sci USA 91, 11884-8.
- Brown, K. et al. (1995) Science 267, 1485-8.
- Brockman, J.A. et al. (1995) Mol Cell Biol 15, 2809-18.
- Traenckner, E.B. et al. (1995) EMBO J 14, 2876-83.
- Chen, Z.J. et al. (1996) Cell 84, 853-62.
- Karin, M. and Ben-Neriah, Y. (2000) Annu Rev Immunol 18, 621-63.
- Hoffmann, A. et al. (2002) Science 298, 1241-5.
- Shirane, M. et al. (1999) J Biol Chem 274, 28169-74.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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