WB, IP
H M R Mk
Endogenous
33
Rabbit IgG
#Q9UK53-2
3621
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human ING1b protein.
Background
Inhibitor of growth 1 (ING1) is a member of an evolutionarily conserved family of tumor suppressor proteins and transcription regulators (1,2). Differential mRNA splicing generates several ING1 isoforms, with the widely expressed ING1b (p33) isoform playing key roles in cell cycle regulation, apoptosis, and senescence (2-5). All ING family proteins contain a plant homeodomain (PHD) that is thought to recognize and bind methylated lysine residues on histone proteins (6,7). The ING1 protein regulates expression of genes through its association with histone acetyltransferase and deacetylase complexes. The PHD of ING1 may facilitate the recruitment of these chromatin-modifying enzymes to targets genes that are regulated by various transcription factors, such as p53 (2, 8-10). Consistent with its role as a tumor suppressor, alterations in ING1 expression levels and cytoplasm localization have been observed in several cancers but mutations in the corresponding ING1 gene in cancers are uncommon (11-14).
- Unoki, M. et al. (2009) Cancer Sci 100, 1173-9.
- Nouman, G.S. et al. (2003) J Clin Pathol 56, 491-6.
- Helbing, C.C. et al. (1997) Cancer Res 57, 1255-8.
- Shinoura, N. et al. (1999) Cancer Res 59, 5521-8.
- Abad, M. et al. (2011) Aging Cell 10, 158-71.
- Garkavtsev, I. et al. (1996) Nat Genet 14, 415-20.
- He, G.H. et al. (2005) Mol Biol Evol 22, 104-16.
- Pedeux, R. et al. (2005) Mol Cell Biol 25, 6639-48.
- Kuzmichev, A. et al. (2002) Mol Cell Biol 22, 835-48.
- Peña, P.V. et al. (2008) J Mol Biol 380, 303-12.
- Toyama, T. et al. (1999) Oncogene 18, 5187-93.
- Ohmori, M. et al. (1999) Am J Hematol 62, 118-9.
- Tallen, G. et al. (2004) Int J Cancer 109, 476-9.
- Li, X.H. et al. (2011) Histol Histopathol 26, 597-607.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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