WB, IP, IF-IC
H
Endogenous
90
Rabbit
#P18084
3693
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| Immunofluorescence (Immunocytochemistry) | 1:200 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys789 of human integrin β5 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
Integrins are α/β heterodimeric cell surface receptors that play a pivotal role in cell adhesion and migration, as well as in growth and survival (1,2). The integrin family contains at least 18 α and 8 β subunits that form 24 known integrins having distinct tissue distribution and overlapping ligand specificities (3). Integrins not only transmit signals to cells in response to the extracellular environment (outside-in signaling), but also sense intracellular cues to alter their interaction with extracellular environment (inside-out signaling) (1,2).
The αVβ5 integrin is expressed in various tissues and cell types, including endothelia, epithelia and fibroblasts (4). It plays a role in matrix adhesion to VN, FN, SPARC and bone sialoprotein (5) and functions in the invasion of gliomas and metastatic carcinoma cells (6,7). αVβ5 integrin plays a major role in growth-factor-induced tumor angiogenesis, where cooperative signaling by the αVβ5 integrin and growth factors regulates endothelial cell proliferation and survival (8).
- Hood, J.D. and Cheresh, D.A. (2002) Nat. Rev. Cancer 2, 91-100.
- Liu, S. et al. (2000) J. Cell Sci. 113, 3563-3571.
- van der Flier, A. and Sonnenberg, A. (2001) Cell Tissue Res. 305, 285-298.
- Pasqualini, R. et al. (1993) J. Cell Sci. 105, 101-111.
- Plow, E.F. et al. (2000) J. Biol. Chem. 275, 21785-21788.
- Sun, L.C. et al. (2007) Cancer Lett. 246, 157-166.
- Enns, A. et al. (2005) Eur. J. Cancer 41, 1065-1072.
- Alghisi, G.C. and Rüegg, C. (2006) Endothelium 13, 113-135.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation IF-IC: Immunofluorescence (Immunocytochemistry)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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