WB
M R
Endogenous
40
Rabbit
#Q60766
15944
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Mouse, Rat
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg215 of mouse IRGM protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
Immunity-related GTPase family M protein 1 (IRGM, LRG-47) belongs to the p47 family of immunity related guanosine triphosphatases (IRGs) that regulate innate immune responses to intracellular pathogens (1-3). Research studies indicate that IRGM plays a role in autophagy during clearance of intracellular bacteria (4). Expression of IRGM in mice, but not in humans, is induced by inflammatory signals that include interferon and LPS (2,3). Polymorphisms in the corresponding IRGM gene are associated with some cases of tuberculosis (5-7), Crohn’s disease (8,9), and severe sepsis (10). Additional studies indicate that IRGM functions through regulation of autophagy (4). Mitochondrial IRGM plays a role in mitochondrial fission, membrane polarization, and mitophagy (11). Knockout mice for IRGM show increased susceptibility to infection as well as intestinal inflammation and Paneth cell abnormalities (12,13). Knockout mice against IRGM are also resistant to neuronal autophagy following stroke (14). RNA viruses commonly target IRGM in order to suppress autophagy and enhance infection (15).
- Kim, B.H. et al. (2012) Cell Host Microbe 12, 432-44.
- Gilly, M. and Wall, R. (1992) J Immunol 148, 3275-81.
- Sorace, J.M. et al. (1995) J Leukoc Biol 58, 477-84.
- Singh, S.B. et al. (2006) Science 313, 1438-41.
- Che, N. et al. (2010) Clin Chim Acta 411, 1645-9.
- Intemann, C.D. et al. (2009) PLoS Pathog 5, e1000577.
- King, K.Y. et al. (2011) PLoS One 6, e16317.
- Parkes, M. et al. (2007) Nat Genet 39, 830-2.
- McCarroll, S.A. et al. (2008) Nat Genet 40, 1107-12.
- Kimura, T. et al. (2014) PLoS One 9, e91522.
- Singh, S.B. et al. (2010) Nat Cell Biol 12, 1154-65.
- Feng, C.G. et al. (2004) J Immunol 172, 1163-8.
- Liu, B. et al. (2013) Am J Physiol Gastrointest Liver Physiol 305, G573-84.
- He, S. et al. (2012) Autophagy 8, 1621-7.
- Grégoire, I.P. et al. (2011) PLoS Pathog 7, e1002422.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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