WB, IP
H M
Endogenous
35, 70
Rabbit
#Q9Y257
9424
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human KCNK6 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
Potassium channels are protein complexes that form potassium ion (K+)-selective pores allowing the passive transport of K+ through biological membranes. They regulate K+ homeostasis and cell volume, and also have physiological functions involved in neurotransmitter and hormone secretion and muscular and neuronal excitability. Many potassium channel proteins have been identified and classified into different groups, mainly based on their membrane topology. The KCNK subfamily (Potassium channel subfamily K) is characterized by the presence of four transmembrane segments (4TMS) and the two-pore (2P) domain (4TMS/2P). These two-pore domain K+ (K2p) channels are considered as leaky channels that maintain background K+ currents to stabilize the negative membrane potential and counterbalance depolarization (1-4).
KCNK6, also known as TWIK-2, is expressed in all human tissues examined except the skeletal muscle (5). A recent study identified KCNK6 as the K+ channel mediating K+ efflux across the plasma membrane that is essential for NLRP3 inflammasome activation (6).
- Goldstein, S.A. et al. (2001) Nat Rev Neurosci 2, 175-84.
- Kim, D. and Kang, D. (2015) Pflugers Arch 467, 1001-11.
- Enyedi, P. and Czirják, G. (2010) Physiol Rev 90, 559-605.
- Mathie, A. et al. (2021) Annu Rev Pharmacol Toxicol, 61:401-420.
- Chavez, R.A. et al. (1999) J Biol Chem 274, 7887-92.
- Di, A. et al. (2018) Immunity 49, 56-65.e4.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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