WB, IF-IC
H M R
Endogenous
110
Rabbit IgG
#P33176
3799
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunofluorescence (Immunocytochemistry) | 1:400 - 1:800 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Phe33 of human KIF5B protein.
Background
Kinesin superfamily proteins (KIFs) are molecular motors that drive directional, microtubule-dependent intracellular transport of membrane-bound organelles and other macromolecules (e.g. proteins, nucleic acids). The intracellular transport functions of KIFs are fundamentally important for a variety of cellular functions, including mitotic and meiotic division, motility/migration, hormone and neurotransmitter release, and differentiation (1-4). Disruptions to KIF-mediated intracellular transport have been linked with a variety of pathologies, ranging from tumorigenesis to defects in higher order brain function such as learning and memory (4-6).
KIF5 consists of three family members referred to as KIF5A, KIF5B, and KIF5C (7). KIF5A and KIF5C are specifically expressed in neurons whereas KIF5B is expressed ubiquitously (7-10). Targeted disruption of the kif5B gene resulted in embryonic lethality (11). These and other studies demonstrate that KIF5B plays an important role in the localization and distribution of major organelles, including the mitochondria and lysosome, and can contribute to autophagy (11-13). In addition, gene rearrangements involving KIF5B fusions to ALK and RET have been identified as drivers for lung cancer and other malignancies (14-18).
- Hirokawa, N. et al. (2009) Nat Rev Mol Cell Biol 10, 682-96.
- Yu, Y. and Feng, Y.M. (2010) Cancer 116, 5150-60.
- Park, J.J. et al. (2008) Mol Endocrinol 22, 989-1005.
- Hirokawa, N. et al. (2010) Neuron 68, 610-38.
- Yoshimura, Y. et al. (2010) Mol Cell Biol 30, 2206-19.
- Hirokawa, N. and Noda, Y. (2008) Physiol Rev 88, 1089-118.
- Nakagawa, T. et al. (1997) Proc Natl Acad Sci U S A 94, 9654-9.
- Aizawa, H. et al. (1992) J Cell Biol 119, 1287-96.
- Kanai, Y. et al. (2000) J Neurosci 20, 6374-84.
- Meng, Y.X. et al. (1997) Endocrinology 138, 1979-87.
- Tanaka, Y. et al. (1998) Cell 93, 1147-58.
- Cardoso, C.M. et al. (2009) PLoS One 4, e4424.
- Du, W. et al. (2016) Dev Cell 37, 326-336.
- Takeuchi, K. et al. (2009) Clin Cancer Res 15, 3143-9.
- Wong, D.W. et al. (2011) Cancer 117, 2709-18.
- Takeuchi, K. et al. (2012) Nat Med 18, 378-81.
- Ju, Y.S. et al. (2012) Genome Res 22, 436-45.
- Kohno, T. et al. (2012) Nat Med 18, 375-7.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IF-IC: Immunofluorescence (Immunocytochemistry)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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