| Product Includes | Product # | Quantity | Mol. Wt | Isotype/Source |
|---|---|---|---|---|
| Phospho-HSL (Ser563) Antibody | 4139 | 20 µl | 81, 83 kDa | Rabbit |
| Phospho-HSL (Ser565) Antibody | 4137 | 20 µl | 81, 83 kDa | Rabbit |
| Perilipin-1 (D1D8) XP® Rabbit mAb | 9349 | 20 µl | 62 kDa | Rabbit IgG |
| Anti-rabbit IgG, HRP-linked Antibody | 7074 | 100 µl | Goat | |
| HSL (D6W5S) XP® Rabbit mAb | 18381 | 20 µl | 81, 83 kDa | Rabbit IgG |
| Phospho-HSL (Ser660) Antibody | 45804 | 20 µl | 81, 83 kDa | Rabbit |
Please visit cellsignal.com for individual component applications, species cross-reactivity, dilutions, protocols, and additional product information.
Description
The Lipolysis Activation Antibody Sampler Kit provides an economical means to evaluate the activation status of multiple members of the lipolysis pathway, including phosphorylated HSL and perilipin. The kit includes enough antibody to perform two western mini-blot experiments with each primary antibody.
Storage
Background
Triacylglycerol is stored in lipid droplets as a primary energy reserve. During lipolysis, triacylglycerols in adipocytes are hydrolyzed into free fatty acids and glycerol. Perilipin, localized at the periphery of lipid droplets, serves as a protective coating against lipases (1-3). Evidence suggests that PKA regulates lipolysis by phosphorylating perilipin and hormone-sensitive lipase (HSL) (1,2,4,5). Phosphorylation of perilipin results in the conformational change that exposes lipid droplets to endogenous lipases, such as HSL (2). Phosphorylation of HSL at Ser563, Ser659, and Ser660 by PKA stimulates HSL activity, which in turn catalyzes the hydrolysis of triacylglycerol (6,7).
- Greenberg, A.S. et al. (1991) J Biol Chem 266, 11341-6.
- Brasaemle, D.L. (2007) J Lipid Res 48, 2547-59.
- Ducharme, N.A. and Bickel, P.E. (2008) Endocrinology 149, 942-9.
- Egan, J.J. et al. (1990) J Biol Chem 265, 18769-75.
- Brasaemle, D.L. et al. (2009) Mol Cell Biochem 326, 15-21.
- Degerman, E. et al. (1990) Proc Natl Acad Sci U S A 87, 533-7.
- Anthonsen, M.W. et al. (1998) J Biol Chem 273, 215-21.
Background References
Trademarks and Patents
Limited Uses
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