Upstream / Downstream

pathwayImage

Explore pathways related to this product.

Dec 31st Santa Cruz will discontinue a
large number of polyclonal products as
a result of the USDA settlement that was
made public May 19th.

Find CST Equivalent

Questions?

Find answers on our FAQs page.

ANSWERS  

PhosphoSitePlus® Resource

  • Additional protein information
  • Analytical tools

LEARN MORE

Product Includes Quantity Applications Reactivity MW(kDa) Isotype
HSL Antibody 4107 20 µl
Western Blotting Immunoprecipitation Immunofluorescence
M 81, 83 Rabbit 
Phospho-HSL (Ser563) Antibody 4139 20 µl
Western Blotting Immunofluorescence
M 81, 83 Rabbit 
Phospho-HSL (Ser565) Antibody 4137 20 µl
Western Blotting Immunofluorescence
M 81, 83 Rabbit 
Phospho-HSL (Ser660) Antibody 4126 20 µl
Western Blotting
M R 81, 83 Rabbit 
Perilipin (D1D8) XP® Rabbit mAb 9349 20 µl
Western Blotting Immunoprecipitation Immunohistochemistry Immunofluorescence
H M 62 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
Western Blotting
Goat 

Product Description

The Lipolysis Activation Antibody Sampler Kit provides an economical means to evaluate the activation status of multiple members of the lipolysis pathway, including phosphorylated HSL and perilipin. The kit includes enough antibody to perform two western mini-blot experiments with each primary antibody.


Specificity / Sensitivity

Each antibody in the Lipolysis Activation Antibody Sampler Kit detects endogenous levels of the respective target protein. Phospho-HSL (Ser563) Antibody does not cross-react with Ser565 phosphorylated HSL. Phospho-HSL (Ser565) Antibody does not cross-react with Ser563 phosphorylated HSL. Phospho-HSL (Ser660) Antibody does not cross-react with Ser563, Ser565, or Ser659 phosphorylated HSL.


Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser552 of human HSL (equivalent to Ser563 of rat HSL), Ser554 of human HSL (equivalent to Ser565 of rat HSL), Ser651 of mouse HSL (equivalent to Ser660 of rat HSL), or the sequence of human HSL. Polyclonal antibodies are purified by protein A and peptide affinity chromatography. Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues around Ile419 of human perilipin protein.

Triacylglycerol is stored in lipid droplets as a primary energy reserve. During lipolysis, triacylglycerols in adipocytes are hydrolyzed into free fatty acids and glycerol. Perilipin, localized at the periphery of lipid droplets, serves as a protective coating against lipases (1-3). Evidence suggests that PKA regulates lipolysis by phosphorylating perilipin and hormone-sensitive lipase (HSL) (1,2,4,5). Phosphorylation of perilipin results in the conformational change that exposes lipid droplets to endogenous lipases, such as HSL (2). Phosphorylation of HSL at Ser563, Ser659, and Ser660 by PKA stimulates HSL activity, which in turn catalyzes the hydrolysis of triacylglycerol (6,7).


1.  Degerman, E. et al. (1990) Proc. Natl. Acad. Sci. USA 87, 533-37.

2.  Anthonsen, M.W. et al. (1998) J. Biol. Chem. 273, 215-21.

3.  Greenberg, A.S. et al. (1991) J Biol Chem 266, 11341-6.

4.  Brasaemle, D.L. (2007) J Lipid Res 48, 2547-59.

5.  Ducharme, N.A. and Bickel, P.E. (2008) Endocrinology 149, 942-9.

6.  Egan, J.J. et al. (1990) J Biol Chem 265, 18769-75.

7.  Brasaemle, D.L. et al. (2009) Mol Cell Biochem 326, 15-21.


Entrez-Gene Id 3991 , 5346
Swiss-Prot Acc. Q05469 , O60240


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

8334
Lipolysis Activation Antibody Sampler Kit