WB
Vir
Endogenous
40
Rabbit
#G8EFI2
11293538
Product Information
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Virus
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro47 of Lloviu virus VP35 protein. Antibodies are purified by peptide affinity chromatography.
Background
The Lloviu virus (LLOV) is a pathogenic filovirus identified in bat populations in southern Europe (1-3). It belongs to the Filoviridae family, members of which (e.g., Ebola, Marburg) are known to cause fatal hemorrhagic diseases in primates, including humans (4). Transcription and replication of filoviruses requires formation of a ribonucleoprotein (RNP) complex, composed of nucleoprotein (NP) together with viral polymerase enzymes and cofactors. Viral Protein 35 (VP35), a polymerase cofactor, is one of the key components of the RNP complex in filoviruses (5). In addition to its role in promoting viral replication, research studies in both bat and human cells have shown that Lloviu virus VP35 can suppress the host innate immune response to viral infection, in part through suppression of the interferon response (6). These immunosuppressive functions of Lloviu virus VP35 are consistent with those observed for VP35 homologs in other filovirus species (7,8).
- Kuhn, J.H. et al. (2010) Arch Virol 155, 2083-103.
- Negredo, A. et al. (2011) PLoS Pathog 7, e1002304.
- Kemenesi, G. et al. (2022) Nat Commun 13, 1706.
- Barrette, R.W. et al. (2011) Infect Genet Evol 11, 1514-9.
- Feagins, A.R. and Basler, C.F. (2015) Virology 485, 145-52.
- Shu, T. et al. (2019) Nucleic Acids Res 47, 5837-5851.
- Basler, C.F. et al. (2003) J Virol 77, 7945-56.
- Hume, A. and Mühlberger, E. (2018) J Infect Dis 218, S403-S408.
- Hume, A.J. et al. (2022) PLoS Pathog 18, e1010268.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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