Cat. # | Size | Qty. | Price |
---|---|---|---|
72219S | 100 µl |
|
REACTIVITY | Vir |
SENSITIVITY | Endogenous |
MW (kDa) | 40 |
SOURCE | Rabbit |
Product Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
NOTE: Please refer to primary antibody product webpage for recommended antibody dilution.
From sample preparation to detection, the reagents you need for your Western Blot are now in one convenient kit: #12957 Western Blotting Application Solutions Kit
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
Load 20 µl onto SDS-PAGE gel (10 cm x 10 cm).
NOTE: Loading of prestained molecular weight markers (#59329, 10 µl/lane) to verify electrotransfer and biotinylated protein ladder (#7727, 10 µl/lane) to determine molecular weights are recommended.
NOTE: Volumes are for 10 cm x 10 cm (100 cm2) of membrane; for different sized membranes, adjust volumes accordingly.
* Avoid repeated exposure to skin.
posted June 2005
revised June 2020
Protocol Id: 10
Virus
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro47 of Lloviu virus VP35 protein. Antibodies are purified by peptide affinity chromatography.
The Lloviu virus (LLOV) is a pathogenic filovirus identified in bat populations in southern Europe (1-3). It belongs to the Filoviridae family, members of which (e.g., Ebola, Marburg) are known to cause fatal hemorrhagic diseases in primates, including humans (4). Transcription and replication of filoviruses requires formation of a ribonucleoprotein (RNP) complex, composed of nucleoprotein (NP) together with viral polymerase enzymes and cofactors. Viral Protein 35 (VP35), a polymerase cofactor, is one of the key components of the RNP complex in filoviruses (5). In addition to its role in promoting viral replication, research studies in both bat and human cells have shown that Lloviu virus VP35 can suppress the host innate immune response to viral infection, in part through suppression of the interferon response (6). These immunosuppressive functions of Lloviu virus VP35 are consistent with those observed for VP35 homologs in other filovirus species (7,8).
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