| Product Includes | Product # | Quantity | Mol. Wt | Isotype/Source |
|---|---|---|---|---|
| G9a/EHMT2 (C6H3) Rabbit mAb | 3306 | 20 µl | 160,180 kDa | Rabbit IgG |
| ESET (C1C12) Rabbit mAb | 2196 | 20 µl | 180 kDa | Rabbit IgG |
| ASH2L (D93F6) XP® Rabbit mAb | 5019 | 20 µl | 80, 65 kDa | Rabbit IgG |
| RBBP5 (D3I6P) Rabbit mAb | 13171 | 20 µl | 70 kDa | Rabbit IgG |
| SET7/SET9 Antibody | 2813 | 20 µl | 48 kDa | Rabbit |
| SMYD2 (D14H7) Rabbit mAb | 9734 | 20 µl | 49 kDa | Rabbit IgG |
| SUV39H1 (D11B6) Rabbit mAb | 8729 | 20 µl | 48 kDa | Rabbit IgG |
| SET8 (C18B7) Rabbit mAb | 2996 | 20 µl | 43 kDa | Rabbit IgG |
| Anti-rabbit IgG, HRP-linked Antibody | 7074 | 100 µl | Goat |
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Description
The Lysine Methyltransferase Antibody Sampler Kit provides a fast and economical means to evaluate endogenous levels of lysine methyltransferases. The kit contains enough primary antibody to perform two western blot experiments per primary antibody.
Storage
Background
SET domain-containing proteins are potential histone methyltransferases (HMTases), which are classified into subgroups by their putative substrate specificities. Histone H3 Lys9 (H3-K9) methyltransferase group genes include Suv39h1, Suv39h2, G9a, G9a related protein (GLP) and SETDB1/ESET (1). The H3-K9 methylation mark plays an important role as a binding site for the chromo-containing protein, resulting in chromatin compaction and heterochromatin generation (2). Histone H3-K4 methylation is exclusively associated with actively transcribed genes (2). The first H3-K4 methylase complex, COMPASS, was identified in the yeast S. cerevisiae and consists of Set1/KMT2 and seven other polypeptides, Cps60-Cps15 (2). Set1/KMT2 functions within COMPASS and is capable of mono-, di-, and trimethylating H3-K4 (2). There are several Set1 related proteins in mammals including WDR5, RBBP5, ASH2L, CXXC1, and DPY30 (2,3). SET7/SET9 is a member of the SET domain-containing family that can specifically methylate H3-K4, Lys189 of the TAF10, a member of the TFIID transcription factor complex, and Lys372 of the p53 tumor suppressor protein (4-6). SET domain-containing lysine methyltransferase 8 (SET8), also known as PR/SET domain-containing protein 7 (PR/SET7), is a single-subunit enzyme that mono-methylates histone H4-K20, preferably on nucleosomal substrates (7-9). SET and MYND domain-containing protein 2 (SMYD2), also known as lysine methyltransferase protein 3C (KMT3C), functions to repress transcription by interacting with the Sin3A repressor complex and methylating H3-K36 (10). SMYD2 also methylates H3-K4 through interaction with HSP90α, and methylates p53 at Lys370 to repress p53-mediated transcriptional activation and apoptosis (11,12).
- Tachibana, M. et al. (2005) Genes Dev 19, 815-26.
- Shilatifard, A. (2008) Curr Opin Cell Biol 20, 341-8.
- Lee, J.H. et al. (2007) J Biol Chem 282, 13419-28.
- Nishioka, K. et al. (2002) Genes Dev 16, 479-89.
- Kouskouti, A. et al. (2004) Mol Cell 14, 175-82.
- Chuikov, S. et al. (2004) Nature 432, 353-60.
- Fang, J. et al. (2002) Curr Biol 12, 1086-99.
- Xiao, B. et al. (2005) Genes Dev 19, 1444-54.
- Couture, J.F. et al. (2005) Genes Dev 19, 1455-65.
- Brown, M.A. et al. (2006) Mol Cancer 5, 26.
- Abu-Farha, M. et al. (2008) Mol Cell Proteomics 7, 560-72.
- Huang, J. et al. (2006) Nature 444, 629-32.
Background References
Trademarks and Patents
Limited Uses
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