Interested in promotions? | Click here >>
8694
Lysine Methyltransferase Antibody Sampler Kit
Primary Antibodies
Antibody Sampler Kit

Lysine Methyltransferase Antibody Sampler Kit #8694

Reviews ()
Citations (0)
Lysine Methyltransferase Antibody Sampler Kit: Image 1

Chromatin immunoprecipitations were performed with cross-linked chromatin from K562 cells and either G9a/EHMT2 (C6H3) or Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human ZNF19 Intron 3 Primers #98428, human VRK3 intron 11 primers, and SimpleChIP® Human GAPDH Exon 1 Primers #5516. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

Lysine Methyltransferase Antibody Sampler Kit: Image 2

Confocal immunofluorescent analysis of MCF-7 cells using ESET (C1C12) Rabbit mAb (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red).

Lysine Methyltransferase Antibody Sampler Kit: Image 3

Confocal imunofluorescent analysis of HeLa cells using ASH2L (D93F6) XP® Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red).

Lysine Methyltransferase Antibody Sampler Kit: Image 4

Chromatin immunoprecipitations were performed with cross-linked chromatin from HeLa cells and either RBBP5 (D3I6P) or Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human CDKN1B Promoter Primers #11951, human CDKN2C promoter primers, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

Lysine Methyltransferase Antibody Sampler Kit: Image 5

Confocal immunofluorescent analysis of HeLa cells using SET7/SET9 Antibody (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red).

Lysine Methyltransferase Antibody Sampler Kit: Image 6

Western blot analysis of extracts from various cell lines using SMYD2 (D14H7) Rabbit mAb.

Lysine Methyltransferase Antibody Sampler Kit: Image 7

Western blot analysis of extracts from various cell lines using SUV39H1 (D11B6) Rabbit mAb.

Lysine Methyltransferase Antibody Sampler Kit: Image 8

Confocal immunofluorescent analysis of HeLa cells, untreated (left) or treated with 0.5% Triton X-100 for 10 minutes prior to fixation according to Tardat et al. (2007) J. Cell Biol. 179, 1413-26 (right), using SET8 (C18B7) Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).

Lysine Methyltransferase Antibody Sampler Kit: Image 9

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

Lysine Methyltransferase Antibody Sampler Kit: Image 10

Confocal immunofluorescent analysis of HeLa cells using G9a/EHMT2 (C6H3) Rabbit mAb (green). Actin filaments have been labeled with DY-555 phalloidin (red).

Lysine Methyltransferase Antibody Sampler Kit: Image 11

Western blot analysis of cell lysates from MCF7 and 293 cells using ESET (C1C12) Rabbit mAb.

Lysine Methyltransferase Antibody Sampler Kit: Image 12

Western blot analysis of extracts from various cell lines using ASH2L (D93F6) XP® Rabbit mAb.

Lysine Methyltransferase Antibody Sampler Kit: Image 13

Immunoprecipitation of RBBP5 from C2C12 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or RBBP5 (D3I6P) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using RBBP5 (D3I6P) Rabbit mAb.

Lysine Methyltransferase Antibody Sampler Kit: Image 14

Western blot analysis of cell lysates from HeLa, NIH/3T3, C6 and COS cells using SET7/SET9 Antibody.

Lysine Methyltransferase Antibody Sampler Kit: Image 15

Western blot analysis of extracts from HCT116 and RAW cells using SET8 (C18B7) Rabbit mAb.

Lysine Methyltransferase Antibody Sampler Kit: Image 16

Western blot analysis of extracts from HeLa and 293 cells using G9a/EHMT2 (C6H3) Rabbit mAb.

Lysine Methyltransferase Antibody Sampler Kit: Image 17

Western blot analysis of extracts from various cell lines using RBBP5 (D3I6P) Rabbit mAb.

To Purchase # 8694T
Product # Size Price
8694T
1 Kit  (8 x 20 µl) $ 554

Our US Office is Closed

Our US office is closed in observance of Thanksgiving. We will reopen on Monday, November 30th.

Thank you for your patience.

Product Includes Quantity Applications Reactivity MW(kDa) Isotype
G9a/EHMT2 (C6H3) Rabbit mAb 3306 20 µl
  • WB
  • IF
  • ChIP
H M R Mk 160,180 Rabbit IgG
ESET (C1C12) Rabbit mAb 2196 20 µl
  • WB
  • IP
  • IF
H Mk 180 Rabbit IgG
ASH2L (D93F6) XP® Rabbit mAb 5019 20 µl
  • WB
  • IP
  • IF
H M R Mk 80, 65 Rabbit IgG
RBBP5 (D3I6P) Rabbit mAb 13171 20 µl
  • WB
  • IP
  • ChIP
H M R Mk 70 Rabbit IgG
SET7/SET9 Antibody 2813 20 µl
  • WB
  • IF
H M R Mk 48 Rabbit 
SMYD2 (D14H7) Rabbit mAb 9734 20 µl
  • WB
  • IP
H M R Mk 49 Rabbit IgG
SUV39H1 (D11B6) Rabbit mAb 8729 20 µl
  • WB
  • IP
H M R Mk 48 Rabbit IgG
SET8 (C18B7) Rabbit mAb 2996 20 µl
  • WB
  • IF
H M R Mk 43 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
  • WB
Goat 

Product Description

The Lysine Methyltransferase Antibody Sampler Kit provides a fast and economical means to evaluate endogenous levels of lysine methyltransferases. The kit contains enough primary antibody to perform two western blot experiments per primary antibody.

Specificity / Sensitivity

Each antibody in this kit recognizes only the specific target protein and does not cross-react with other family members.

Source / Purification

Monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human ASH2L protein, the carboxy terminus of human ESET protein, the carboxy terminus of human G9a/EHMT2 protein, human SET8 protein, residues surrounding Val414 of human SMYD2 protein, residues surrounding Val408 of human RBBP5 protein, or residues surrounding Asp380 of human SUV39H1 protein. Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues the human SET7/SET9 protein. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.

Background

SET domain-containing proteins are potential histone methyltransferases (HMTases), which are classified into subgroups by their putative substrate specificities. Histone H3 Lys9 (H3-K9) methyltransferase group genes include Suv39h1, Suv39h2, G9a, G9a related protein (GLP) and SETDB1/ESET (1). The H3-K9 methylation mark plays an important role as a binding site for the chromo-containing protein, resulting in chromatin compaction and heterochromatin generation (2). Histone H3-K4 methylation is exclusively associated with actively transcribed genes (2). The first H3-K4 methylase complex, COMPASS, was identified in the yeast S. cerevisiae and consists of Set1/KMT2 and seven other polypeptides, Cps60-Cps15 (2). Set1/KMT2 functions within COMPASS and is capable of mono-, di-, and trimethylating H3-K4 (2). There are several Set1 related proteins in mammals including WDR5, RBBP5, ASH2L, CXXC1, and DPY30 (2,3). SET7/SET9 is a member of the SET domain-containing family that can specifically methylate H3-K4, Lys189 of the TAF10, a member of the TFIID transcription factor complex, and Lys372 of the p53 tumor suppressor protein (4-6). SET domain-containing lysine methyltransferase 8 (SET8), also known as PR/SET domain-containing protein 7 (PR/SET7), is a single-subunit enzyme that mono-methylates histone H4-K20, preferably on nucleosomal substrates (7-9). SET and MYND domain-containing protein 2 (SMYD2), also known as lysine methyltransferase protein 3C (KMT3C), functions to repress transcription by interacting with the Sin3A repressor complex and methylating H3-K36 (10). SMYD2 also methylates H3-K4 through interaction with HSP90α, and methylates p53 at Lys370 to repress p53-mediated transcriptional activation and apoptosis (11,12).

  1. Tachibana, M. et al. (2005) Genes Dev 19, 815-26.
  2. Shilatifard, A. (2008) Curr Opin Cell Biol 20, 341-8.
  3. Lee, J.H. et al. (2007) J Biol Chem 282, 13419-28.
  4. Nishioka, K. et al. (2002) Genes Dev. 16, 479-489.
  5. Kouskouti, A. et al. (2004) Mol. Cell 14, 175-182.
  6. Chuikov, S. et al. (2004) Nature 432, 353-360.
  7. Fang, J. et al. (2002) Curr Biol 12, 1086-99.
  8. Xiao, B. et al. (2005) Genes Dev 19, 1444-54.
  9. Couture, J.F. et al. (2005) Genes Dev 19, 1455-65.
  10. Brown, M.A. et al. (2006) Mol Cancer 5, 26.
  11. Abu-Farha, M. et al. (2008) Mol Cell Proteomics 7, 560-72.
  12. Huang, J. et al. (2006) Nature 444, 629-32.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.