| Product Includes | Product # | Quantity | Mol. Wt | Isotype/Source |
|---|---|---|---|---|
| G9a/EHMT2 (C6H3) Rabbit mAb | 3306 | 20 µl | 160,180 kDa | Rabbit IgG |
| ESET (C1C12) Rabbit mAb | 2196 | 20 µl | 180 kDa | Rabbit IgG |
| ASH2L (D93F6) XP® Rabbit mAb | 5019 | 20 µl | 80, 65 kDa | Rabbit IgG |
| RBBP5 (D3I6P) Rabbit mAb | 13171 | 20 µl | 70 kDa | Rabbit IgG |
| SET7/SET9 Antibody | 2813 | 20 µl | 48 kDa | Rabbit |
| SMYD2 (D14H7) Rabbit mAb | 9734 | 20 µl | 49 kDa | Rabbit IgG |
| SUV39H1 (D11B6) Rabbit mAb | 8729 | 20 µl | 48 kDa | Rabbit IgG |
| SET8 (C18B7) Rabbit mAb | 2996 | 20 µl | 43 kDa | Rabbit IgG |
| Anti-rabbit IgG, HRP-linked Antibody | 7074 | 100 µl | Goat |
Please visit cellsignal.com for individual component applications, species cross-reactivity, dilutions, protocols, and additional product information.
Description
The Lysine Methyltransferase Antibody Sampler Kit provides a fast and economical means to evaluate endogenous levels of lysine methyltransferases. The kit contains enough primary antibody to perform two western blot experiments per primary antibody.
Storage
Background
SET domain-containing proteins are potential histone methyltransferases (HMTases), which are classified into subgroups by their putative substrate specificities. Histone H3 Lys9 (H3-K9) methyltransferase group genes include Suv39h1, Suv39h2, G9a, G9a related protein (GLP) and SETDB1/ESET (1). The H3-K9 methylation mark plays an important role as a binding site for the chromo-containing protein, resulting in chromatin compaction and heterochromatin generation (2). Histone H3-K4 methylation is exclusively associated with actively transcribed genes (2). The first H3-K4 methylase complex, COMPASS, was identified in the yeast S. cerevisiae and consists of Set1/KMT2 and seven other polypeptides, Cps60-Cps15 (2). Set1/KMT2 functions within COMPASS and is capable of mono-, di-, and trimethylating H3-K4 (2). There are several Set1 related proteins in mammals including WDR5, RBBP5, ASH2L, CXXC1, and DPY30 (2,3). SET7/SET9 is a member of the SET domain-containing family that can specifically methylate H3-K4, Lys189 of the TAF10, a member of the TFIID transcription factor complex, and Lys372 of the p53 tumor suppressor protein (4-6). SET domain-containing lysine methyltransferase 8 (SET8), also known as PR/SET domain-containing protein 7 (PR/SET7), is a single-subunit enzyme that mono-methylates histone H4-K20, preferably on nucleosomal substrates (7-9). SET and MYND domain-containing protein 2 (SMYD2), also known as lysine methyltransferase protein 3C (KMT3C), functions to repress transcription by interacting with the Sin3A repressor complex and methylating H3-K36 (10). SMYD2 also methylates H3-K4 through interaction with HSP90α, and methylates p53 at Lys370 to repress p53-mediated transcriptional activation and apoptosis (11,12).
- Tachibana, M. et al. (2005) Genes Dev 19, 815-26.
- Shilatifard, A. (2008) Curr Opin Cell Biol 20, 341-8.
- Lee, J.H. et al. (2007) J Biol Chem 282, 13419-28.
- Nishioka, K. et al. (2002) Genes Dev 16, 479-89.
- Kouskouti, A. et al. (2004) Mol Cell 14, 175-82.
- Chuikov, S. et al. (2004) Nature 432, 353-60.
- Fang, J. et al. (2002) Curr Biol 12, 1086-99.
- Xiao, B. et al. (2005) Genes Dev 19, 1444-54.
- Couture, J.F. et al. (2005) Genes Dev 19, 1455-65.
- Brown, M.A. et al. (2006) Mol Cancer 5, 26.
- Abu-Farha, M. et al. (2008) Mol Cell Proteomics 7, 560-72.
- Huang, J. et al. (2006) Nature 444, 629-32.
Background References
Trademarks and Patents
Limited Uses
Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.
Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.
