WB, IHC-Bond, IHC-P
H M
Endogenous
62
Rabbit IgG
#Q64133
17161
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| IHC Leica Bond | 1:800 - 1:3200 |
| Immunohistochemistry (Paraffin) | 1:800 - 1:3200 |
Storage
Specificity / Sensitivity
This antibody recognizes mouse Maoa protein and is also reactive with human MAOA protein; however, this antibody is not suggested for immunohistochemical analysis of human tissues. Nonspecific staining was observed in mouse testis by immunohistochemistry.
Species Reactivity:
Human, Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala467 of mouse Maoa protein.
Background
Monoamine oxidase A (MAOA), a mitochondrial enzyme, catalyzes the degradation of monoamines. Studies show that increased expression of MAOA promotes prostate cancer metastasis by activating the Shh-IL6-RANKL signaling network (1). In addition, MAOA expression in adipose tissue macrophages is upregulated in aging, leading to the reduction of noradrenaline availability in adipose tissue. Decreased levels of noradrenaline contribute to the aging-related decline of catecholamine-induced lipolysis in adipocytes (2). Furthermore, sympathetic neuron–associated macrophages import and degrade norepinephrine via the transporter SLC6A2 and MAOA, respectively, and thus contribute to obesity (3).
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IHC-Bond: IHC Leica Bond IHC-P: Immunohistochemistry (Paraffin)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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