WB
H M R
Endogenous
47, 49
Rabbit IgG
#P31153
4144
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human MAT2A protein.
Background
Methionine adenosyltransferase II alpha (MAT2A) uses methionine and ATP as substrates to generate S-adenosylmethionine (SAM), a universal methyl group donor in cells. Tumor-initiating cells (TICs) have high methionine cycle activity mediated by MAT2A, leading to their metabolic dependency on exogenous methionine (1). Studies show that mTORC1 signaling induces MAT2A expression, leading to elevated synthesis of SAM. In addition, mTORC1 increases the protein level of Wilms' tumor 1-associating protein (WTAP), a positive regulatory subunit of the human N6-methyladenosine (m6 A) RNA methyltransferase complex. mTORC1, therefore, promotes N6-methylation of mRNA to stimulate protein synthesis and cell growth (2). Furthermore, inhibition of MAT2A activity was shown to decrease proliferation of cancer cells deficient in methylthioadenosine phosphorylase (MTAP) (3).
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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