Cell Signaling Technology Logo
View Featured Offers >>
1% for the planet logo
  1. Products /
  2. Primary Antibodies /
  3. MAT2A (E5I7C) Rabbit mAb
Trial Size Available Flag
Recombinant Flag
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

MAT2A (E5I7C) Rabbit mAb #84478

Filter:
  • WB

    Supporting Data

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 47, 49
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    MAT2A (E5I7C) Rabbit mAb recognizes endogenous levels of total MAT2A protein. This antibody does not cross-react with MAT1A protein.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human MAT2A protein.

    Background

    Methionine adenosyltransferase II alpha (MAT2A) uses methionine and ATP as substrates to generate S-adenosylmethionine (SAM), a universal methyl group donor in cells. Tumor-initiating cells (TICs) have high methionine cycle activity mediated by MAT2A, leading to their metabolic dependency on exogenous methionine (1). Studies show that mTORC1 signaling induces MAT2A expression, leading to elevated synthesis of SAM. In addition, mTORC1 increases the protein level of Wilms' tumor 1-associating protein (WTAP), a positive regulatory subunit of the human N6-methyladenosine (m6A) RNA methyltransferase complex. mTORC1, therefore, promotes N6-methylation of mRNA to stimulate protein synthesis and cell growth (2). Furthermore, inhibition of MAT2A activity was shown to decrease proliferation of cancer cells deficient in methylthioadenosine phosphorylase (MTAP) (3).

    Alternate Names

    AdoMet synthase 2; adoMet synthetase 2; AMS2; MAT 2; MAT-II; MAT2A; MATA2; MATII; Methionine adenosyltransferase 2; methionine adenosyltransferase 2A; Methionine adenosyltransferase II; methionine adenosyltransferase II, alpha; METK2; S-adenosylmethionine synthase isoform type-2; S-adenosylmethionine synthetase isoform type-2; SAMS2; testicular tissue protein Li 121

    Database Links

    UniProt ID: P31153


    Entrez-Gene Id: 4144


    Limited Uses

    Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

    Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

    For Research Use Only. Not for Use in Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    All other trademarks are the property of their respective owners. Visit our Trademark Information page.