Cat. # | Size | Qty. | Price |
---|---|---|---|
84478S | 100 µl |
|
REACTIVITY | H M R |
SENSITIVITY | Endogenous |
MW (kDa) | 47, 49 |
Source/Isotype | Rabbit IgG |
Product Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
NOTE: Please refer to primary antibody product webpage for recommended antibody dilution.
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
Load 20 µl onto SDS-PAGE gel (10 cm x 10 cm).
NOTE: Loading of prestained molecular weight markers (#59329, 10 µl/lane) to verify electrotransfer and biotinylated protein ladder (#7727, 10 µl/lane) to determine molecular weights are recommended.
NOTE: Volumes are for 10 cm x 10 cm (100 cm2) of membrane; for different sized membranes, adjust volumes accordingly.
* Avoid repeated exposure to skin.
posted June 2005
revised June 2020
Protocol Id: 263
Human, Mouse, Rat
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human MAT2A protein.
Methionine adenosyltransferase II alpha (MAT2A) uses methionine and ATP as substrates to generate S-adenosylmethionine (SAM), a universal methyl group donor in cells. Tumor-initiating cells (TICs) have high methionine cycle activity mediated by MAT2A, leading to their metabolic dependency on exogenous methionine (1). Studies show that mTORC1 signaling induces MAT2A expression, leading to elevated synthesis of SAM. In addition, mTORC1 increases the protein level of Wilms' tumor 1-associating protein (WTAP), a positive regulatory subunit of the human N6-methyladenosine (m6 A) RNA methyltransferase complex. mTORC1, therefore, promotes N6-methylation of mRNA to stimulate protein synthesis and cell growth (2). Furthermore, inhibition of MAT2A activity was shown to decrease proliferation of cancer cells deficient in methylthioadenosine phosphorylase (MTAP) (3).
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