WB, IP
H
Endogenous
48-52
Rabbit IgG
#Q96FL8
55244
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu505 of human MATE1 protein.
Background
The multidrug and toxin extrusion protein 1 (MATE1, SLC47A1) is a proton-coupled, organic cation antiporter located at the apical membrane of proximal kidney epithelial cells and the canalicular membrane of hepatocytes (1). MATE1 mediates the secretion of organic cations including drugs, toxins, and endogenous metabolites, into bile and urine (2,3). Substrates of MATE1 include multiple therapeutic agents, including metformin, cisplatin, acyclovir, and cephalexin (4,5). Polymorphisms in the corresponding SLC47A1 gene may affect the rate of renal clearance of certain cationic drugs, limiting the therapeutic benefits of these agents (6). Specifically, research studies demonstrate that SLC47A1 allelic variation correlates with differences in renal clearance rates of metformin (7), which may have an effect on the therapeutic impact of this drug in individuals diagnosed with type 2 diabetes (8).
- Otsuka, M. et al. (2005) Proc Natl Acad Sci U S A 102, 17923-8.
- Omote, H. et al. (2006) Trends Pharmacol Sci 27, 587-93.
- Motohashi, H. and Inui, K. (2013) AAPS J 15, 581-8.
- Tanihara, Y. et al. (2007) Biochem Pharmacol 74, 359-71.
- Hume, W.E. et al. (2013) Bioorg Med Chem 21, 7584-90.
- Staud, F. et al. (2013) Int J Biochem Cell Biol 45, 2007-11.
- Christensen, M.M. et al. (2013) Pharmacogenet Genomics 23, 526-34.
- Becker, M.L. et al. (2009) Diabetes 58, 745-9.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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