WB, IF-F, IF-IC
H M R
Endogenous
28
Rabbit
#P27573
17528
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunofluorescence (Frozen) | 1:1600 |
| Immunofluorescence (Immunocytochemistry) | 1:50 - 1:200 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val218 of mouse Myelin Protein Zero protein. Antibodies are purified by peptide affinity chromatography.
Background
Myelin Protein Zero (MPZ, myelin P0) is a single pass membrane glycoprotein member of the IgG superfamily (1,2). MPZ is the most abundant myelin protein in the peripheral nervous system and acts as a major structural component of the peripheral myelin sheath (2,3). It has been shown that MPZ is necessary for normal myelin spiraling, compaction, and maintenance, with disruption of the MPZ gene leading to hypomyelination and degradation of associated axons (4).
Mutations in the MPZ gene have been associated with demyelinating peripheral neuropathies including Charcot-Marie-Tooth neuropathy type 1B (CMT1B), Dejerine–Sottas syndrome (DSS), and congenital hypomyelination (CH) (5,6).
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IF-F: Immunofluorescence (Frozen) IF-IC: Immunofluorescence (Immunocytochemistry)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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