WB, IP
H M R
Endogenous
210
Rabbit IgG
#Q92859
4756
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ile1250 of human neogenin protein.
Background
Neogenin is a member of the Deleted in Colorectal Cancer (DCC) receptor family. It acts as an attractive axon guidance receptor in response to netrin, and as a repellent receptor when interacting with RGMa (1,2). Neogenin also regulates adhesion and tissue organization, such as bone formation, mammary gland morphogenesis, and skeletal myofiber development, by interacting with secreted netrins (3-5). In addition, neogenin is a key regulator of adult neurogenesis by synchronizing neuroblast migration and differentiation (6). Neogenin expression has been found to be associated with cellular phenotype in cancer. In breast cancer, neogenin expression is inversely correlated with cancer grade (7), while in gastric cancer, up regulation of neogenin was found to increase cell proliferation and motility (8).
- Rajagopalan, S. et al. (2004) Nat Cell Biol 6, 756-62.
- Wilson, B.D. et al. (2006) Science 313, 640-4.
- Kang, J.S. et al. (2004) J Cell Biol 167, 493-504.
- Srinivasan, K. et al. (2003) Dev Cell 4, 371-82.
- Hagihara, M. et al. (2011) J Biol Chem 286, 5157-65.
- O'Leary, C.J. et al. (2015) Stem Cells 33, 503-14.
- Xing, W. et al. (2014) World J Surg Oncol 12, 352.
- Kim, S.J. et al. (2014) Oncotarget 5, 3386-98.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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