WB
H M R
Endogenous
58
Rabbit IgG
#Q9UBE8
51701
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human NLK protein.
Background
Nemo-like kinase (NLK ) is a serine/threonine-protein kinase that regulates multiple signaling pathways, including Wnt/β-catenin, TGFβ, IL-6, and Notch (1-4). NLK contributes to cell proliferation, differentiation, cell fate determination during early embryogenesis and nervous system development in vertebrates (5-7). Recent studies showed that NLK is aberrantly expressed in various types of cancer where it regulates cancer cell proliferation, migration, invasion and survival (8-11). NLK is localized predominantly in nucleus and at a lower level in cytoplasm(12). Homodimerization of NLK is required for its activation and nuclear localization. NLK is activated via intermolecular autophosphorylation at Thr286 (13). NLK interacts with and phosphorylates a number of transcription factors including FOXO1, FOXO4, MYB, NOTCH1 and TCF7L2/TCF4, and LEF-1/TCF (14-18). NLK also associates with E3 ubiquitin ligase NARF and Raptor and regulates their function (19,20).
- Smit, L. et al. (2004) J Biol Chem 279, 17232-40.
- Ohkawara, B. et al. (2004) Genes Dev 18, 381-6.
- Kojima, H. et al. (2005) Proc Natl Acad Sci U S A 102, 4524-9.
- Ishitani, T. et al. (2010) Nat Cell Biol 12, 278-85.
- Hyodo-Miura, J. et al. (2002) Genes Cells 7, 487-96.
- Thorpe, C.J. and Moon, R.T. (2004) Development 131, 2899-909.
- Satoh, K. et al. (2007) Mol Cell Biol 27, 7623-30.
- Li, M. et al. (2013) Tumour Biol 34, 3995-4000.
- Lv, L. et al. (2014) J Cell Biochem 115, 81-92.
- Huang, Y. et al. (2013) PLoS One 8, e69148.
- Dong, J.R. et al. (2013) Asian Pac J Cancer Prev 14, 7137-41.
- Brott, B.K. et al. (1998) Proc Natl Acad Sci U S A 95, 963-8.
- Ishitani, S. et al. (2011) Mol Biol Cell 22, 266-77.
- Ishitani, T. et al. (2003) Mol Cell Biol 23, 1379-89.
- Kanei-Ishii, C. et al. (2004) Genes Dev 18, 816-29.
- Kim, S. et al. (2010) J Biol Chem 285, 8122-9.
- Togi, S. et al. (2011) J Biol Chem 286, 19170-7.
- Szypowska, A.A. et al. (2011) Antioxid Redox Signal 14, 563-78.
- Yamada, M. et al. (2006) J Biol Chem 281, 20749-60.
- Yuan, H.X. et al. (2015) Genes Dev 29, 2362-76.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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