WB, IHC-P, IF-F, IF-IC
H M R Mk
Endogenous
100
Rabbit IgG
#Q08J23
54888
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunohistochemistry (Paraffin) | 1:200 - 1:800 |
| Immunofluorescence (Frozen) | 1:1600 - 1:3200 |
| Immunofluorescence (Immunocytochemistry) | 1:3200 - 1:12800 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human NSUN2 protein.
Background
Chemical modifications of RNA regulate many cellular processes. One particular RNA modification, 5-methylcytosine (5-mC), regulates ribosome assembly, translation, and RNA stability (1). In eukaryotes, this modification is added to RNA by the DNA methyltransferase homologue 2 protein (DNMT2; also known as TRDMT1), and also by members of the NOL1/NOP2/SUN domain (NSUN) family of proteins. NSUN proteins are putative S-adenosylmethionine (SAM)-dependent methyltransferases that carry out their enzymatic activity by utilizing two cysteine residues in their active sites (2). There are currently seven known members of this family, consisting of NOP2 (NSUN1) and NSUN2-7.
NSUN2 is an 86 kDa member of the NSUN family that is predominantly localized to the nucleus and methylates a variety of tRNAs and other RNA substrates (3). NSUN2 has been found to play a role in the methylation of pre-tRNALeu, tRNAGly, tRNAVal, tRNALeu, and tRNAAsp (1,4,5). It has also been found to methylate mRNAs and several ncRNAs, such as vtRNAs (6-8). Elevated NSUN2 protein levels have been reported in multiple types of human cancers, including breast, prostate, kidney, bladder, and liver, among others (9). The methyltransferase activity of NSUN2 is reduced upon Aurora B kinase-mediated phosphorylation at Ser139 (10), and there is potential for NSUN2 to be used as a therapeutic target or diagnostic marker for Aurora B linked cancers (9).
- Trixl, L. and Lusser, A. (2019) Wiley Interdiscip Rev RNA 10, e1510.
- Bohnsack, K.E. et al. (2019) Genes (Basel) 10, pii: E102. doi: 10.3390/genes10020102.
- Bourgeois, G. et al. (2015) PLoS One 10, e0133321.
- Brzezicha, B. et al. (2006) Nucleic Acids Res 34, 6034-43.
- Tuorto, F. et al. (2012) Nat Struct Mol Biol 19, 900-5.
- Tang, H. et al. (2015) Aging (Albany NY) 7, 1143-58.
- Xing, J. et al. (2015) Mol Cell Biol 35, 4043-52.
- Hussain, S. et al. (2013) Cell Rep 4, 255-61.
- Okamoto, M. et al. (2012) DNA Cell Biol 31, 660-71.
- Sakita-Suto, S. et al. (2007) Mol Biol Cell 18, 1107-17.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IHC-P: Immunohistochemistry (Paraffin) IF-F: Immunofluorescence (Frozen) IF-IC: Immunofluorescence (Immunocytochemistry)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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