| Product Includes | Product # | Quantity | Mol. Wt | Isotype/Source |
|---|---|---|---|---|
| CHD3 Antibody | 4241 | 20 µl | 260 kDa | Rabbit |
| CHD4 (D8B12) Rabbit mAb | 11912 | 20 µl | 260 kDa | Rabbit IgG |
| HDAC1 (10E2) Mouse mAb | 5356 | 20 µl | 62 kDa | Mouse IgG1 |
| HDAC2 (3F3) Mouse mAb | 5113 | 20 µl | 60 kDa | Mouse IgG1 |
| MBD3 (N87) Antibody | 14540 | 20 µl | 32, 34 kDa | Rabbit |
| MTA1 (D40D1) XP® Rabbit mAb | 5647 | 20 µl | 78-82 kDa | Rabbit IgG |
| RBAP46 (V415) Antibody | 6882 | 20 µl | 48 kDa | Rabbit |
| Anti-rabbit IgG, HRP-linked Antibody | 7074 | 100 µl | Goat | |
| Anti-mouse IgG, HRP-linked Antibody | 7076 | 100 µl | Horse |
Please visit cellsignal.com for individual component applications, species cross-reactivity, dilutions, protocols, and additional product information.
Description
NuRD Complex Antibody Sampler Kit offers an economical means of detecting each target protein that composes the nucleosome remodeling and deacetylation complex (NuRD). The kit contains enough primary antibody to perform two western blot experiments with each primary antibody.
Storage
Background
The transcriptional repressor NuRD (nucleosome remodeling and histone deacetylase) complex is composed of multiple subunits, which include CHD3 (also known as Mi2-α), CHD4 (also known as Mi2-β), HDAC1, HDAC2, RBAP48, RBAP46, MTA1, MTA2, MTA3, and MBD3 (1-6). Both CHD3 and CHD4 contain two PHD (plant homeodomain) zinc finger domains that bind directly to HDAC1 and HDAC2. HDAC1 and HDAC2 are highly homologous and are involved in histone deacetylation, chromatin remodeling and transcriptional repression, and are also found together in the mSin3A and MeCP1 complexes (7-9). RBAP46 and RBAP48 bind to the histone fold region of histone H4 and are believed to target the NuRD complex to its histone substrates (10). MBD3, a member of the methyl-CpG-binding domain protein family, and the metastasis associated genes MTA1, MTA2, and MTA3 were all found to be integral components of the NuRD complex (2,3,7,11).
- Tong, J.K. et al. (1998) Nature 395, 917-21.
- Xue, Y. et al. (1998) Mol Cell 2, 851-61.
- Zhang, Y. et al. (1998) Cell 95, 279-89.
- Bowen, N.J. et al. (2004) Biochim Biophys Acta 1677, 52-7.
- Jones, P.L. et al. (1998) Nat Genet 19, 187-91.
- Fujita, N. et al. (2003) Cell 113, 207-19.
- Zhang, Y. et al. (1999) Genes Dev 13, 1924-35.
- Ng, H.H. et al. (1999) Nat Genet 23, 58-61.
- Zhang, Y. et al. (1997) Cell 89, 357-64.
- Verreault, A. et al. (1998) Curr Biol 8, 96-108.
- Wade, P.A. et al. (1999) Nat Genet 23, 62-6.
Background References
Trademarks and Patents
Limited Uses
Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.
Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.
